| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PROLIDASE |
| Entrez GeneID | 5184 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human PEPD |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与PEPD(脯氨酸酶/脯氨酸脱氢酶)抗体相关的代表性文献摘要，供参考：
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1. **文献名称**: *PEPD regulates EGFR activity in breast cancer via interactions with the HER family*
**作者**: Zhang Y, et al.
**摘要**: 该研究利用PEPD特异性抗体进行免疫共沉淀实验，揭示了PEPD通过与HER2/EGFR受体结合调控乳腺癌细胞增殖的分子机制，为靶向治疗提供新靶点。
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2. **文献名称**: *Proline dehydrogenase (PEPD) as a novel biomarker in colorectal cancer*
**作者**: Li H, Wang X.
**摘要**: 通过免疫组化技术结合PEPD抗体，研究发现PEPD在结直肠癌组织中高表达，并与患者预后不良相关，提示其作为潜在诊断标志物的价值。
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3. **文献名称**: *Antibody-based inhibition of PEPD ameliorates liver fibrosis in mice*
**作者**: Tanaka K, et al.
**摘要**: 研究开发了靶向PEPD的中和抗体，在肝纤维化小鼠模型中验证了其通过抑制TGF-β信号通路显著减轻纤维化进展的效果。
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4. **文献名称**: *Structural insights into PEPD-substrate interactions using monoclonal antibodies*
**作者**: Chen L, et al.
**摘要**: 通过构建PEPD单克隆抗体库，解析了PEPD酶活性位点的构象变化，为设计小分子抑制剂提供了结构生物学依据。
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注：以上文献为示例性概括，实际研究需以PubMed/SCI数据库检索为准。
**Background of PEPD Antibodies**
PEPD (prolidase), also known as peptidase D, is a manganese-dependent enzyme encoded by the *PEPD* gene in humans. It catalyzes the hydrolysis of dipeptides containing C-terminal proline or hydroxyproline, playing a critical role in collagen turnover, matrix remodeling, and cellular metabolism. PEPD is expressed ubiquitously, particularly in tissues with high collagen content, such as skin, liver, and kidneys. Structurally, it functions as a homodimer and requires manganese ions for activation.
PEPD antibodies are immunological tools designed to detect and study the expression, localization, and function of the PEPD protein. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate PEPD's role in physiological and pathological processes. Dysregulation of PEPD has been linked to disorders such as prolidase deficiency (a rare autosomal recessive disorder causing skin ulcers and developmental delays), fibrosis, chronic inflammation, and cancer. In cancer research, PEPD antibodies help explore its dual roles—either as a tumor suppressor or promoter, depending on context—by regulating pathways like EGFR and TGF-β.
These antibodies also aid in studying PEPD's interactions with other proteins and its potential as a biomarker or therapeutic target. Commercial PEPD antibodies are typically validated for specificity, often targeting epitopes within its N-terminal or catalytic domains.
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