| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WNT3A |
| Uniprot No | P56704-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-385aa |
| 氨基酸序列 | MAPLGYFLLLCSLKQALGSYPIWWSLAVGPQYSSLGSQPILCASIPGLVP KQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGP VLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGW KWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMH LKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESR GWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVS SHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHT CKNPGSRAGNSAHQPPHPQPPVRFHPPLRRAGKVP |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于WNT3A重组蛋白的参考文献及其摘要概括：
1. **文献名称**：*"Wnt proteins are lipid-modified and can act as stem cell growth factors"*
**作者**：Willert K, et al.
**摘要**：该研究报道了从哺乳动物细胞(如HEK293T)中纯化具有生物活性的重组Wnt3a蛋白的方法，并证明其通过脂质修饰维持活性，能够促进干细胞自我更新和β-catenin信号通路激活。
2. **文献名称**：*"Maintenance of pluripotency in human embryonic stem cells by Wnt3a-conditioned medium"*
**作者**：Sato N, et al.
**摘要**：研究显示，重组Wnt3a蛋白的条件培养基可替代传统饲养层细胞，维持人胚胎干细胞的未分化状态，其作用依赖于经典Wnt/β-catenin信号通路的激活。
3. **文献名称**：*"Autocrine WNT3A signaling promotes tumor progression in colorectal cancer"*
**作者**：Krishnamurthy N, Kuriyama S, et al.
**摘要**：该文献揭示结直肠癌细胞通过自分泌WNT3A蛋白激活下游信号通路，促进肿瘤增殖和侵袭，提示WNT3A重组蛋白在癌症模型研究中的潜在应用价值。
4. **文献名称**：*"Recombinant production of biologically active Wnt3a in HEK293 cells"*
**作者**：Zhang Y, et al.
**摘要**：本文优化了在HEK293细胞中高效表达和纯化功能性重组Wnt3a蛋白的流程，验证其在小鼠肝脏干细胞分化中的活性，为体外研究Wnt信号机制提供可靠工具。
(注：以上信息基于领域内典型研究概括，具体文献需通过数据库核实。)
**Background of WNT3A Recombinant Protein**
WNT3A, a member of the Wnt protein family, is a secreted glycoprotein critical for regulating cell signaling pathways involved in embryonic development, tissue homeostasis, and stem cell maintenance. It specifically activates the canonical Wnt/β-catenin signaling cascade by binding to Frizzled receptors and LRP co-receptors, stabilizing β-catenin, and promoting its nuclear translocation to regulate target gene expression.
Recombinant WNT3A is produced using advanced biotechnological methods, often via mammalian expression systems (e.g., HEK293 cells), ensuring proper post-translational modifications like glycosylation, which are essential for its biological activity. Purification techniques such as affinity chromatography yield high-purity, bioactive proteins.
WNT3A is widely utilized in research to study tissue regeneration, stem cell self-renewal, and differentiation. It is a key tool for maintaining pluripotency in embryonic stem cells and inducing organoid formation. Dysregulation of WNT3A is linked to cancers, fibrosis, and developmental disorders, making it a focus for therapeutic exploration.
As a research reagent, recombinant WNT3A provides a standardized, scalable alternative to endogenous proteins, enabling consistent experimental outcomes in developmental biology and disease modeling.
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