| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WLS |
| Uniprot No | Q5T9L3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 101-232aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEMSPWF QFMLFILQLD IAFKLNNQIR ENAEVSMDVS LAYRDDAFAE WTEMAHERVP RKLKCTFTSP KTPEHEGRYY ECDVLPFMEI GSVAHKFYLL NIRLPVNEKK KINVGIGEIK DIRLVGIHQN GGFTK |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WLS(Wntless)重组蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
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1. **文献名称**：*Expression and Purification of Recombinant WLS Protein in Mammalian Cells*
**作者**：Smith A, et al.
**摘要**：本研究利用HEK293细胞系统成功表达了重组WLS蛋白，优化了其分泌纯化条件，并验证了其在体外促进Wnt3a蛋白分泌的功能活性，为Wnt信号通路研究提供了工具。
2. **文献名称**：*Structural Insights into WLS-Mediated Wnt Transport by Cryo-EM*
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜技术解析了重组WLS蛋白与Wnt8的复合物结构，揭示了WLS如何结合并协助Wnt蛋白的跨膜转运，阐明了其分子机制。
3. **文献名称**：*Prokaryotic Expression of Truncated WLS and Its Role in Colorectal Cancer*
**作者**：Wang Y, et al.
**摘要**：在大肠杆菌中高效表达并纯化了截短型WLS重组蛋白，实验证明其可抑制结直肠癌细胞中异常Wnt信号传导，提示潜在治疗应用。
4. **文献名称**：*WLS Knockout Cell Lines and Rescue with Recombinant WLS*
**作者**：Kim T, et al.
**摘要**：构建了WLS基因敲除的细胞模型，并通过外源重组WLS蛋白回补实验证实其在Wnt分泌中的必要性，为功能研究提供新模型。
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注：以上文献为模拟示例，实际研究中建议通过PubMed、Google Scholar等平台检索具体文献。
**Background of WLS Recombinant Protein**
WLS (Wntless), also known as GPR177. is a conserved transmembrane protein critical for the secretion and trafficking of Wnt signaling proteins, which play pivotal roles in embryonic development, tissue homeostasis, and stem cell regulation. Discovered in genetic studies of *Drosophila*, WLS acts as a dedicated chaperone for Wnt ligands by binding to their palmitoleated residues, ensuring their proper post-translational modification, stabilization, and release from producing cells. Dysregulation of WLS-mediated Wnt secretion is linked to developmental disorders, cancers, and degenerative diseases, highlighting its biological and therapeutic significance.
Recombinant WLS protein refers to the engineered form of WLS produced in heterologous expression systems (e.g., mammalian, insect, or bacterial cells) for research or therapeutic applications. Its recombinant production enables scalable, high-purity yields, facilitating structural and functional studies. Researchers utilize WLS recombinant protein to investigate Wnt secretion mechanisms, screen for modulators of Wnt signaling, or develop targeted therapies. For instance, in cancer research, inhibiting WLS-Wnt interactions may suppress oncogenic signaling.
Structurally, WLS contains eight transmembrane domains and a conserved Wnt-binding region at its C-terminus. Recombinant versions often include tags (e.g., His, GST) for purification and detection. Challenges in production include preserving its native conformation and lipid-modified Wnt-binding capacity, often requiring eukaryotic expression systems.
Current studies focus on WLS’s role in tissue regeneration, drug resistance, and as a biomarker. Its recombinant form also serves as a tool in organoid cultures and regenerative medicine to enhance Wnt-dependent protocols. Overall, WLS recombinant protein bridges basic research and translational applications, offering insights into Wnt biology and therapeutic strategies targeting this pathway.
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