| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABHD12B |
| Uniprot No | Q7Z5M8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-255aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMLGIWHTVPSCRGEDAKGKDCCWYEAA LRDGNPIIVYLHGSAEHRAASHRLKLVKVLSDGGFHVLSVDYRGFGDSTG KPTEEGLTTDAICVYEWTKARSGITPVCLWGHSLGTGVATNAAKVLEEKG CPVDAIVLEAPFTNMWVASINYPLLKIYRNIPGFLRTLMDALRKDKIIFP NDENVKFLSSPLLILHGEDDRTVPLEYGKKLYEIARNAYRNKERVKMVIF PPGFQHNLLCKSPTLLITVRDFLSKQWS |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABHD12B重组蛋白的参考文献示例(注：部分内容为假设性概括，实际文献可能需要根据具体研究补充)：
---
1. **标题**: "Recombinant Expression and Enzymatic Characterization of ABHD12B in Lipid Metabolism"
**作者**: Smith J, et al.
**摘要**: 本研究在大肠杆菌系统中成功表达并纯化了重组ABHD12B蛋白，通过体外酶活实验证实其具有溶血磷脂酰丝氨酸(lyso-PS)水解活性，提示ABHD12B可能参与神经系统的脂质信号传导调控。
2. **标题**: "Structural Insights into ABHD12B and Its Role in Neurodegenerative Disorders"
**作者**: Lee S, et al.
**摘要**: 通过杆状病毒-昆虫细胞系统制备重组ABHD12B蛋白，结合X射线晶体学解析其三维结构，发现其活性位点与ABHD12存在差异，可能解释两者在底物选择性和相关神经疾病(如PHARC综合征)中的不同作用。
3. **标题**: "ABHD12B Recombinant Protein as a Therapeutic Target in Glioblastoma"
**作者**: Johnson R, et al.
**摘要**: 利用哺乳动物细胞表达的重组ABHD12B蛋白进行高通量药物筛选，发现其抑制剂可降低胶质母细胞瘤细胞的增殖能力，表明ABHD12B可能成为癌症治疗的潜在靶点。
4. **标题**: "Comparative Analysis of ABHD12 and ABHD12B Substrate Specificities Using Recombinant Proteins"
**作者**: Martinez L, et al.
**摘要**: 研究通过共表达ABHD12和ABHD12B重组蛋白，结合质谱分析，揭示两者在甘油磷脂代谢中的不同底物偏好性，为ABHD家族蛋白的功能分化提供实验依据。
---
**注意**：以上文献为示例性内容，实际研究中ABHD12B的相关文献可能有限，建议通过PubMed、Google Scholar等平台以关键词“ABHD12B recombinant protein”或“ABHD12B expression”检索最新进展。若研究较少，可扩展至ABHD家族或同源蛋白(如ABHD12)的文献进行参考。
ABHD12B (α/β-Hydrolase Domain-Containing Protein 12B) is a member of the serine hydrolase superfamily, characterized by a conserved α/β-hydrolase fold. Although its exact physiological role remains unclear, ABHD12B shares structural homology with ABHD12. a lysophospholipase implicated in endocannabinoid metabolism and neurological disorders. ABHD12B is predicted to hydrolyze lipid substrates, potentially regulating bioactive lipid signaling pathways. However, unlike ABHD12. which is associated with neurodegenerative conditions like PHARC syndrome (polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataracts), ABHD12B has not been definitively linked to human diseases. Its gene is conserved across vertebrates, suggesting evolutionary significance.
Recombinant ABHD12B protein is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Researchers utilize purified ABHD12B to investigate its enzymatic activity, substrate specificity, and interaction partners. Epitope tags (e.g., His-tag) are often added to facilitate purification and detection. Current studies focus on elucidating its potential role in lipid metabolism, particularly in contexts like neuroinflammation or cancer, where lipid signaling pathways are dysregulated. Challenges include characterizing its endogenous substrates and clarifying functional distinctions from ABHD12. Advances in structural biology (e.g., crystallography) may help decipher its catalytic mechanism and guide therapeutic targeting. As an understudied hydrolase, ABHD12B represents a frontier in lipid enzymology with implications for both basic biology and translational research.
×
