首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠α-黑色素细胞刺激素(α-MSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的α-MSH含量。α - MSH即α - 促黑素细胞激素，它是一种内源性神经肽。背景上，其在体内多种生理调节中起重要作用。研究机制方面，它能与相应受体结合，参与调节黑素生成、能量代谢、免疫反应及炎症调控等过程，是当前生物学和医学领域的研究热点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于体外实验模型中α-MSH分泌水平的动态监测，例如评估药物干预对神经内分泌通路的影响、炎症模型中肽类激素变化分析，或黑色素细胞功能相关的基础研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：alpha-melanocyte stimulating hormone, alpha-MSH
缩写：α-MSH
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse α-MSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of mouse α-MSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.8732.8012.8372.6784002.2682.2452.2572.0982001.6541.5581.6061.4471000.8970.8670.8820.723500.5810.5620.5720.413250.3870.3660.3770.21812.50.2980.2840.2910.13200.1610.1570.159 | ||||||
货期：3-5 working days
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