| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPS6KB2; STK14B; Ribosomal protein S6 kinase beta-2; S6K-beta-2; S6K2; 70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta; S6K-beta; p70 |
| Entrez GeneID | 6199 |
| WB Predicted band size | Calculated MW: 53 kDa; Observed MW: 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human P70 S6 Kinase beta |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于S6K2抗体的代表性文献概览(内容为模拟生成，非真实文献)：
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1. **"Characterization of S6K2-specific antibodies for detecting isoform-specific mTOR signaling"**
*作者：Li, X. et al.*
摘要：本研究开发了针对S6K2蛋白C端结构域的高特异性单克隆抗体，验证了其在Western blot和免疫组化中对S6K2与S6K1的区分能力，揭示了S6K2在乳腺癌细胞中独特的亚细胞定位及mTORC2调控机制。
2. **"S6K2 phosphorylation dynamics in neuronal development revealed by phospho-specific antibodies"**
*作者：Garcia-Martinez, J.M. & Alessi, D.R.*
摘要：通过构建针对S6K2 Thr398/Ser401磷酸化位点的抗体，研究团队在小鼠脑组织中发现S6K2的活化模式与突触可塑性密切相关，该抗体为神经发育疾病中mTOR信号异常研究提供了关键工具。
3. **"Isoform-specific roles of S6K in metabolism: Insights from S6K2 knockout mice and antibody-based tissue profiling"**
*作者：Um, S.H. et al.*
摘要：利用特异性识别S6K2的抗体进行组织分布分析，发现S6K2在肝脏脂代谢中的独特作用，其抗体检测结果支持S6K2通过IRS-1调控胰岛素敏感性，与S6K1功能形成互补。
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注：如需真实文献，建议在PubMed数据库以"S6K2 antibody"或"RPS6KB2 antibody"为关键词检索，并筛选涉及抗体开发验证或特异性应用的研究。
The S6K2 antibody is a crucial tool for studying the ribosomal protein S6 kinase beta-2 (S6K2), a serine/threonine kinase encoded by the *RPS6KB2* gene. S6K2. along with its isoform S6K1. is a downstream effector of the mTOR (mechanistic target of rapamycin) signaling pathway, which regulates cell growth, proliferation, and survival in response to nutrient availability and growth signals. While S6K1 is predominantly cytosolic, S6K2 localizes to both the nucleus and cytoplasm, suggesting distinct roles in transcriptional regulation and translation.
S6K2 antibodies are widely used in research to detect and quantify S6K2 protein expression, phosphorylation status (e.g., at Thr395/Thr404), and activation dynamics. These antibodies are essential for techniques like Western blotting, immunohistochemistry, and immunofluorescence, enabling insights into mTOR pathway activity in diseases such as cancer, metabolic disorders, and neurodegenerative conditions. Specific S6K2 antibodies help differentiate its functions from S6K1. particularly in contexts like tumor progression, where S6K2 has been implicated in chemoresistance and cell cycle regulation.
Commercial S6K2 antibodies are typically raised against synthetic peptides corresponding to unique regions of the human S6K2 protein. Validation includes testing for specificity against recombinant proteins or knockout cell lines. Researchers rely on these antibodies to explore S6K2’s role in cellular stress responses, autophagy, and its potential as a therapeutic target in precision medicine.
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