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Rabbit Monoclonal p73 Antibody

  • 中文名: p73抗体
  • 别    名: TP73; P73; Tumor protein p73; p53-like transcription factor; p53-related protein
货号: IPDX21798
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesTP73; P73; Tumor protein p73; p53-like transcription factor; p53-related protein
Entrez GeneID7161
WB Predicted band sizeCalculated MW: 70 kDa; Observed MW: 80,70 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthetic peptide of human p73
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3条关于p73抗体的参考文献及其摘要概括:

1. **文献名称**:*"Differential expression of TAp73 and ΔNp73 isoforms in human cancers: implications for prognosis"*

**作者**:Schmidte et al.

**摘要**:该研究通过特异性p73抗体(区分TAp73和ΔNp73亚型)分析多种肿瘤组织,发现ΔNp73高表达与患者预后不良相关,提示其在肿瘤进展中的关键作用。

2. **文献名称**:*"Monoclonal antibodies specific for p73: comparative analysis in normal and malignant tissues"*

**作者**:Moll et al.

**摘要**:开发并验证了针对p73蛋白不同表位的单克隆抗体,证实其在免疫组化(IHC)和Western blot中的高特异性,并发现p73在卵巢癌中异常高表达。

3. **文献名称**:*"p73 function is inhibited by tumor-derived p53 mutants in mammalian cells"*

**作者**:Kartasheva et al.

**摘要**:利用p73特异性抗体研究p73与p53突变体的相互作用,发现某些p53突变体能抑制p73的转录活性,揭示了肿瘤中p53-p73通路异常的机制。

4. **文献名称**:*"p63 and p73 expression in developing and adult nervous systems"*

**作者**:Urist et al.

**摘要**:通过特异性抗体标记,系统分析p73蛋白在小鼠神经系统发育中的时空表达模式,发现其在神经元分化中具有重要调控功能。

(注:以上文献信息为示例,实际引用需以真实文献为准。)

背景信息

The p73 antibody is a crucial tool in molecular biology and cancer research, targeting the p73 protein, a member of the p53 tumor suppressor family. Discovered in 1997. p73 shares structural and functional similarities with p53. including roles in cell cycle regulation, apoptosis, and DNA repair. However, unlike p53. which is frequently mutated in cancers, p73 is rarely mutated but often dysregulated through overexpression or isoform imbalance. The p73 gene produces two major isoforms via alternative promoters: TAp73 (pro-apoptotic) and ΔNp73 (anti-apoptotic), which exert opposing biological effects. p73 antibodies are specifically designed to distinguish these isoforms and their post-translational modifications, enabling researchers to study their distinct roles in development, tissue homeostasis, and tumorigenesis. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess p73 expression patterns in normal tissues and malignancies. Their application has revealed p73's context-dependent functions, including neural development, immune regulation, and chemosensitivity in cancer. However, challenges remain in ensuring antibody specificity due to high homology between p73 and its family members, particularly p63. Ongoing research utilizing p73 antibodies continues to explore its potential as a therapeutic target or biomarker in cancers retaining wild-type p53.

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