| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | P4HB; DSI; ERBA2L; GIT; P4Hbeta; PDI; PDIA1; PHDB; PO4DB; PO4HB; PROHB |
| Entrez GeneID | 5034 |
| WB Predicted band size | Calculated MW: 57 kDa; Observed MW: 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human P4HB |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于P4HB抗体的代表性文献摘要(模拟生成，非真实文献)：
1. **文献名称**：*P4HB monoclonal antibody development and its application in detecting hepatocellular carcinoma*
**作者**：Chen L, et al. (2022)
**摘要**：本研究报道了一种新型小鼠源P4HB单克隆抗体的开发，通过免疫印迹和免疫组化验证了其在肝细胞癌组织中的高特异性结合能力，发现P4HB表达水平与患者生存率呈负相关。
2. **文献名称**：*Prolyl 4-hydroxylase beta (P4HB) as a novel biomarker in glioblastoma: Validation using a rabbit polyclonal antibody*
**作者**：Wang Y, et al. (2020)
**摘要**：通过构建兔多克隆抗体，证实P4HB在胶质母细胞瘤细胞系中显著高表达，抗体成功应用于流式细胞术和免疫荧光成像，揭示P4HB通过调控HIF-1α通路促进肿瘤血管生成。
3. **文献名称**：*Antibody-based profiling of P4HB in endoplasmic reticulum stress models*
**作者**：Gupta R, et al. (2019)
**摘要**：利用商业化山羊抗人P4HB抗体(货号ab12345)，系统评估了内质网应激条件下结肠癌细胞中P4HB的亚细胞定位变化，证实其与GRP78/BiP的共定位现象。
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*注：实际文献需通过PubMed/Google Scholar检索关键词“P4HB antibody”+应用领域(如癌症、纤维化等)获取，建议优先选择近5年且经过同行评审的研究。*
The P4HB antibody targets the protein Prolyl 4-Hydroxylase Beta Polypeptide (P4HB), also known as Protein Disulfide Isomerase A1 (PDIA1). P4HB is a multifunctional enzyme primarily located in the endoplasmic reticulum (ER), where it plays critical roles in protein folding, disulfide bond formation, and post-translational modifications of collagens. It acts as both a chaperone and a redox catalyst, ensuring proper tertiary structure and stability of secreted and membrane-bound proteins. Dysregulation of P4HB is linked to ER stress, oxidative stress, and pathologies such as cancer, fibrosis, and neurodegenerative disorders.
P4HB antibodies are widely used in research to study protein quality control mechanisms, ER stress responses, and disease pathways. In cancer biology, P4HB overexpression is associated with tumor progression, metastasis, and chemoresistance, making it a potential therapeutic target or prognostic marker. These antibodies are also employed to validate P4HB’s interaction with hypoxia-inducible factors (e.g., HIF-1α) under low-oxygen conditions. Additionally, due to P4HB’s stable expression across tissues, its antibody is often used as a loading control in Western blotting. Commercial P4HB antibodies are typically developed in rabbit or mouse hosts, with validation in applications like immunofluorescence, immunohistochemistry, and flow cytometry. Recent studies also explore P4HB inhibitors for modulating ER stress in diseases like diabetes and ischemia-reperfusion injury.
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