| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WBP2 |
| Uniprot No | Q969T9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-100aa |
| 氨基酸序列 | MALNKNHSEGGGVIVNNTESILMSYDHVELTFNDMKNVPEAFKGTKKGTV YLTPYRVIFLSKGKDAMQSFMMPFYLMKDCEIKQPVFGANYIKGTVKAEA |
| 预测分子量 | 11 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WBP2重组蛋白的3篇代表性文献及简要摘要：
1. **文献名称**：*WBP2 promotes Yes-associated protein (YAP) transcriptional activity by enhancing its translocation to the nucleus in breast cancer cells*
**作者**：Hong W, Guan KL
**摘要**：该研究通过重组WBP2蛋白的体外实验，发现WBP2通过结合YAP蛋白调控其核转位，激活下游致癌基因的转录，促进乳腺癌细胞增殖和迁移。
2. **文献名称**：*WBP2 modulates Wnt/β-catenin signaling and tumor progression in triple-negative breast cancer*
**作者**：Lim SK, Lu SY, et al.
**摘要**：利用重组WBP2蛋白进行功能研究，证明WBP2通过增强Wnt/β-catenin信号通路的活性，驱动三阴性乳腺癌的侵袭性表型，提示其作为治疗靶点的潜力。
3. **文献名称**：*WW domain-mediated interaction of WBP2 with ERα enhances estrogen signaling in breast cancer*
**作者**：Lee TH, Tan TZ, et al.
**摘要**：研究发现重组WBP2蛋白通过其WW结构域与雌激素受体α(ERα)结合，增强ERα的转录活性，促进乳腺癌细胞对雌激素的依赖性生长和耐药性。
*注：以上文献信息为示例性质，实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science等平台检索最新研究。*
**Background of WBP2 Recombinant Protein**
WBP2 (WW domain-binding protein 2) is a multifunctional adaptor protein involved in regulating cellular processes such as transcriptional activation, signal transduction, and protein-protein interactions. It was initially identified through its ability to interact with WW domain-containing proteins, including the Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), key regulators of the Hippo signaling pathway. Structurally, WBP2 contains two WW domains that mediate binding to proline-rich motifs in partner proteins, as well as a coiled-coil region and a nuclear localization signal, enabling its role in both cytoplasmic and nuclear signaling.
WBP2 has been implicated in cancer progression, particularly in breast and thyroid cancers, where it enhances oncogenic signaling pathways like Wnt/β-catenin and ERK. Its interaction with estrogen receptor alpha (ERα) further links it to hormone-responsive malignancies. Beyond cancer, WBP2 contributes to developmental processes and tissue homeostasis, with studies highlighting its role in mammary gland development and auditory function.
Recombinant WBP2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms of the protein for research. This tool enables *in vitro* studies of WBP2’s interactions, post-translational modifications (e.g., phosphorylation), and mechanistic roles in disease pathways. Researchers also utilize it to screen for inhibitors targeting WBP2-mediated oncogenic activity, positioning it as a potential therapeutic target. The development of WBP2 recombinant protein has thus advanced our understanding of its dual roles in physiology and pathology, offering insights for translational applications.
×
