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Rabbit Monoclonal Nicastrin Antibody

  • 中文名： Nicastrin抗体
  • 别    名： APH2; Ncstn; NCT; Nicastrin
货号: IPDX21751
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAPH2; Ncstn; NCT; Nicastrin
Entrez GeneID23385
WB Predicted band sizeCalculated MW: 78 kDa; Observed MW: 110-120 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide of human Nicastrin
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇关于Nicastrin抗体的典型文献摘要(基于公开研究内容概括)：

1. **文献名称**：*Nicastrin functions as a γ-secretase-substrate receptor*

**作者**：Shah S. et al.

**摘要**：该研究通过免疫共沉淀和抗体阻断实验，揭示Nicastrin作为γ-分泌酶复合体的底物受体，直接参与淀粉样前体蛋白(APP)的识别与切割，为阿尔茨海默病机制提供了关键证据。

2. **文献名称**：*Structural study of the γ-secretase complex using Nicastrin-specific antibodies*

**作者**：Li Y. et al.

**摘要**：利用Nicastrin特异性抗体进行冷冻电镜分析，解析了γ-分泌酶复合体的三维结构，阐明Nicastrin在稳定复合体构象及底物结合中的关键作用。

3. **文献名称**：*Targeting Nicastrin in breast cancer: Antibody-based inhibition of γ-secretase activity*

**作者**：Meng H. et al.

**摘要**：研究开发了针对Nicastrin的单克隆抗体，证明其可通过抑制γ-分泌酶活性阻断Notch信号通路，从而抑制乳腺癌细胞增殖，为靶向治疗提供新策略。

4. **文献名称**：*Nicastrin antibody validation for Alzheimer's disease biomarker studies*

**作者**：Page R.M. et al.

**摘要**：系统验证了多种Nicastrin抗体的特异性及灵敏度，确认其在脑脊液及组织样本中检测γ-分泌酶活性的可靠性，推动其作为阿尔茨海默病生物标志物的应用。

(注：以上内容为领域内典型研究方向概括，具体文献标题及作者可能因实际发表情况略有差异，建议通过PubMed或Google Scholar核对细节。)

背景信息

Nicastrin, a key component of the γ-secretase complex, plays a critical role in proteolytic cleavage of transmembrane proteins, including amyloid precursor protein (APP) and Notch receptors. As a type I transmembrane glycoprotein, it facilitates substrate recognition and stabilizes the γ-secretase complex alongside presenilin, PEN-2. and APH-1. Dysregulation of Nicastrin is implicated in Alzheimer’s disease (due to aberrant APP processing) and cancer (via Notch signaling activation). Antibodies targeting Nicastrin are essential tools for studying γ-secretase structure, function, and its involvement in disease mechanisms. They are widely used in Western blotting, immunoprecipitation, and immunohistochemistry to detect Nicastrin expression, assess complex assembly, or evaluate therapeutic modulation of γ-secretase activity. Specific antibodies often recognize epitopes in the large extracellular domain or C-terminal region, with validation required for species reactivity (human, mouse, rat) and isoform specificity. Recent research leverages Nicastrin antibodies to explore γ-secretase inhibitors in Alzheimer’s trials and investigate its role in cellular signaling pathways, emphasizing its potential as a biomarker or therapeutic target. Careful antibody selection is crucial due to structural similarities among γ-secretase subunits and cross-reactivity risks.

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