| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BATF |
| Uniprot No | Q16520 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-125aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MPHSSDSSDS SFSRSPPPGK QDSSDDVRRV QRREKNRIAA QKSRQRQTQK ADTLHLESED LEKQNAALRK EIKQLTEELK YFTSVLNSHE PLCSVLAAST PSPPEVVYSA HAFHQPHVSS PRFQP |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BATF重组蛋白的3篇参考文献，涵盖功能、结构及应用研究：
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1. **文献名称**：*BATF regulates Th17 differentiation through cooperation with IRF4*
**作者**：Murphy, T.L., et al.
**摘要**：该研究通过重组BATF蛋白实验，揭示了BATF与IRF4转录因子协同调控Th17细胞分化的分子机制，证明其通过结合特定DNA序列影响自身免疫疾病相关基因表达。
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2. **文献名称**：*Structural basis of BATF-mediated DNA recognition in AP-1/IRF complexes*
**作者**：Glasmacher, E., et al.
**摘要**：通过晶体结构分析重组人源BATF蛋白，阐明其碱性亮氨酸拉链(bZIP)结构域与DNA结合的分子细节，为设计靶向BATF的小分子抑制剂提供结构基础。
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3. **文献名称**：*BATF-dependent loss of effector T cell function in chronic infection*
**作者**：Quigley, M., et al.
**摘要**：利用重组BATF蛋白的体外功能实验，发现BATF过表达会抑制CD8+ T细胞抗病毒功能，提示其在慢性感染中通过耗竭T细胞促进免疫逃逸的病理作用。
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(注：以上文献信息为示例性质，实际文献需通过学术数据库验证。)
**Background of BATF Recombinant Protein**
BATF (Basic Leucine Zipper Transcription Factor ATF-like) is a member of the AP-1 transcription factor family, characterized by its leucine zipper domain. Unlike canonical AP-1 proteins (e.g., c-Fos, c-Jun), BATF lacks a canonical DNA-binding domain and often functions as a transcriptional repressor or modulator by forming heterodimers with other AP-1 or interferon regulatory factor (IRF) family members. It plays critical roles in immune regulation, particularly in lymphocyte differentiation, including Th17. T follicular helper (Tfh), and plasma cells, as well as B-cell antibody class switching.
BATF is implicated in autoimmune diseases, allergies, and cancer. In tumors, it exhibits dual roles: promoting immunosuppression in the tumor microenvironment or acting as a tumor suppressor, depending on context. Its dysregulation is linked to aberrant cytokine production (e.g., IL-10. IL-21) and disrupted immune homeostasis.
Recombinant BATF proteins are engineered to study its molecular interactions, signaling pathways, and therapeutic potential. Produced via *E. coli* or mammalian expression systems, these proteins retain functional domains (e.g., leucine zipper) and are purified using affinity tags (e.g., His-tag). Applications include *in vitro* binding assays, structural studies, and screening for inhibitors/activators targeting BATF-mediated pathways.
Research on BATF recombinant proteins aids in unraveling its role in immune disorders and cancer, offering insights for developing targeted therapies. Its unique regulatory mechanisms make it a promising candidate for modulating immune responses in precision medicine.
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