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产品描述：人Wnt抑制因子1(WIF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的WIF1含量。WIF1作为一个靶点，有一定重要生物学意义。它参与了细胞内信号传导通路，可能与肿瘤发生、发展相关。目前研究重点围绕其在调控肿瘤细胞增殖、侵袭、转移等机制，解析其作用机制有望为癌症防治提供新靶点和策略。试剂盒检测范围为47 pg/mL-3000 pg/mL，该产品适用于基础医学研究中Wnt信号通路机制探索、肿瘤微环境分析、骨发育相关疾病模型构建等场景，为体外评估WIF1在细胞间通讯、组织再生及病理状态中的生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：WIF-1 ELISA Kit; wif1 ELISA Kit; WIF1_HUMAN ELISA Kit; Wnt inhibitory factor 1 ELISA Kit
缩写：WIF1
Uniprot No.：Q9Y5W5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Stem Cells
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human WIF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-106 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 87-103 | |||||
回收率：
| The recovery of human WIF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 83-105 | ||||
| EDTA plasma (n=4) | 95 | 87-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.6632.5172.5902.44515001.8251.8541.8401.6957500.9651.0040.9850.8403750.5540.5700.5620.417187.50.3500.3420.3460.201940.2210.2340.2280.083470.1800.1920.1860.04100.1430.1470.145 | ||||||
货期：3-5 working days
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