| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Visfatin |
| Uniprot No | P43490 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-491aa |
| 氨基酸序列 | GPLGSNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTEN SKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVF NEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTN WIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYR GVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAE HSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLR HLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPP YLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLN CSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEG KGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Visfatin重组蛋白的3篇代表性文献，包含标题、作者及摘要核心内容概括：
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1. **标题**：*Recombinant visfatin promotes macrophage apoptosis via the TLR4/JNK signaling pathway*
**作者**：Li Y, et al.
**摘要**：研究利用大肠杆菌表达系统制备重组Visfatin蛋白，并验证其诱导巨噬细胞凋亡的功能，发现其通过激活TLR4/JNK通路调控炎症反应，为代谢相关疾病的机制研究提供依据。
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2. **标题**：*Expression and purification of biologically active human visfatin in Pichia pastoris*
**作者**：Zhang Q, et al.
**摘要**：通过毕赤酵母系统高效表达具有生物活性的重组人Visfatin，优化纯化工艺后获得高纯度蛋白，验证其NAD合成酶活性及在脂肪细胞分化中的作用，为体外功能研究提供可靠工具。
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3. **标题**：*Structural insights into the catalytic mechanism of visfatin/Nampt using recombinant protein crystallography*
**作者**：Kim MK, et al.
**摘要**：解析重组Visfatin蛋白的晶体结构，阐明其催化NAD合成的分子机制，发现关键活性位点突变影响酶活性和胰岛素模拟功能，为靶向药物设计奠定结构基础。
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注：以上文献信息为模拟示例，实际引用时需核对真实来源及原文内容。如需具体文献链接或补充年份/期刊信息，可进一步补充检索。
Visfatin, also known as nicotinamide phosphoribosyltransferase (NAMPT) or pre-B-cell colony-enhancing factor (PBEF), is a multifunctional protein initially identified as a cytokine-like factor involved in immune regulation and later recognized for its critical role in cellular metabolism. It functions as a key enzyme in the NAD+ biosynthesis pathway, catalyzing the conversion of nicotinamide into nicotinamide mononucleotide (NMN), thereby influencing energy metabolism, aging, and stress responses. Elevated visfatin levels are associated with metabolic disorders, inflammation, and cancer progression, making it a biomarker and therapeutic target of interest.
Recombinant visfatin protein is produced using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper folding and post-translational modifications. The purified protein retains enzymatic activity and biological functions, enabling researchers to study its mechanisms in vitro and in vivo. Its recombinant form is widely used to investigate metabolic pathways, insulin-mimetic effects, and interactions with cellular receptors. Studies also explore its dual role as an adipokine contributing to obesity-related insulin resistance and as a pro-inflammatory mediator in chronic diseases.
Current research focuses on visfatin's involvement in diabetes, cardiovascular diseases, and cancer, with recombinant protein tools aiding drug discovery and structural analyses. Challenges remain in clarifying its tissue-specific functions and reconciling contradictory findings regarding its pro- or anti-inflammatory roles. Quality control during production, including activity assays and endotoxin testing, ensures reliability for experimental and potential clinical applications.
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