| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAT1 |
| Uniprot No | Q13838 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-251aa |
| 氨基酸序列 | AENDVDNELLDYEDDEVETAAGGDGAEAPAKKDVKGSYVSIHSSGFRDFLLKPELLRAIVDCGFEHPSEVQHECIPQAILGMDVLCQAKSGMGKTAVFVLATLQQLEPVTGQVSVLVMCHTRELAFQISKEYERFSKYMPNVKVAVFFGGLSIKKDEEVLKKNCPHIVVGTPGRILALARNKSLNLKHIKHFILDECDKMLEQLDMRRDVQEIFRMTPHEKQVMMFSATLSKEIRPVCRKFMQDPMEIFV |
| 预测分子量 | 55.2kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and purification of recombinant BAT1 protein for structural studies"**
- Author: Zhang et al.
- 摘要：本研究描述了通过大肠杆菌表达系统高效表达BAT1重组蛋白的优化方法，并利用亲和层析技术纯化获得高纯度蛋白，为后续结构解析和功能研究奠定基础。
2. **"BAT1 recombinant protein modulates mRNA splicing in vitro"**
- Author: Wang et al.
- 摘要：通过体外实验验证BAT1重组蛋白的RNA解旋酶活性，证明其参与调控mRNA剪接过程，并揭示了其与剪接体复合物的相互作用机制。
3. **"Functional characterization of BAT1 in immune response regulation"**
- Author: Smith et al.
- 摘要：研究发现BAT1重组蛋白通过影响细胞因子mRNA的稳定性，在巨噬细胞的炎症反应中起关键调控作用，为自身免疫疾病治疗提供潜在靶点。
4. **"BAT1 interacts with viral RNA to inhibit replication in host cells"**
- Author: Li et al.
- 摘要：利用重组BAT1蛋白进行病毒-宿主互作研究，发现其通过结合流感病毒RNA抑制病毒复制，提示其在抗病毒治疗中的应用潜力。
注：以上文献信息为示例性内容，实际文献需通过学术数据库检索确认。
**Background of BAT1 Recombinant Protein**
BAT1 (HLA-B associated transcript 1), also known as DDX39B or UAP56. is a member of the DEAD-box RNA helicase family, which plays critical roles in RNA metabolism, including splicing, nuclear export, and translation. It is encoded by the *DDX39B* gene located near the HLA-B locus on chromosome 6. highlighting its potential link to immune regulation. BAT1 interacts with components of the spliceosome and the transcription-export (TREX) complex, facilitating mRNA processing and transport from the nucleus to the cytoplasm.
Studies suggest BAT1 is involved in immune responses, particularly in antigen presentation. By regulating mRNA stability and translation of pro-inflammatory cytokines, it may modulate inflammatory pathways. Its association with HLA-B, a key molecule in adaptive immunity, further implies roles in autoimmune diseases, viral infections, and cancer. For instance, BAT1 polymorphisms have been linked to altered susceptibility to rheumatoid arthritis, HIV progression, and tumor immunogenicity.
Recombinant BAT1 protein is produced using expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical functions, interactions, and therapeutic potential. Purified BAT1 is utilized in *in vitro* assays to dissect RNA helicase activity, screen for inhibitors, or explore its role in immune signaling. Its recombinant form also aids in structural studies, such as crystallography, to map functional domains.
Overall, BAT1 recombinant protein serves as a vital tool for understanding RNA biology, immune regulation, and developing targeted therapies for inflammation-related disorders and cancers.
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