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| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VEGF165 |
| Uniprot No | P15692-4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-191aa |
| 氨基酸序列 | APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPS CVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN KCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQ LELNERTCRCDKPRR |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VEGF165重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Purification and cloning of vascular endothelial growth factor secreted by pituitary folliculostellate cells"*
**作者**：Ferrara, N., Henzel, W.J.
**摘要**：该研究首次报道了从牛垂体细胞中纯化并克隆VEGF165.证实其通过结合VEGFR2受体促进血管内皮细胞增殖和迁移，为后续重组蛋白表达及功能研究奠定基础。
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2. **文献名称**：*"Three-dimensional structure of recombinant human vascular endothelial growth factor165"*
**作者**：Zheng, Y., et al.
**摘要**：通过X射线晶体学解析了重组人VEGF165的三维结构，揭示了其与受体结合的构象特征，为靶向VEGF的药物设计(如抗体疗法)提供了结构生物学依据。
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3. **文献名称**：*"Recombinant VEGF165 administration promotes therapeutic angiogenesis in a murine model of chronic limb ischemia"*
**作者**：Albrecht, S., et al.
**摘要**：在小鼠慢性肢体缺血模型中，重组VEGF165蛋白的局部注射显著增强侧支血管形成，改善血流灌注，证实其作为缺血性疾病的潜在治疗手段。
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4. **文献名称**：*"Efficient production of recombinant human VEGF165 in E. coli and its role in stem cell proliferation"*
**作者**：Lee, J., et al.
**摘要**：开发了一种高效的大肠杆菌表达系统生产功能性VEGF165.并通过实验验证其促进干细胞增殖和分化的活性，为规模化生产及再生医学应用提供技术支持。
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以上文献涵盖VEGF165的分子机制、结构解析、治疗应用及生产方法，均为该领域经典或关键技术研究。
Vascular Endothelial Growth Factor 165 (VEGF165), a predominant isoform of the VEGF family, plays a central role in angiogenesis and vascular permeability. Generated through alternative splicing of the VEGF-A gene, VEGF165 contains 165 amino acids and retains both heparin-binding and receptor-binding domains, enabling interactions with extracellular matrix components and cell surface receptors. It primarily binds to VEGF receptor-1 (VEGFR-1) and VEGFR-2. activating downstream signaling pathways that promote endothelial cell survival, proliferation, and migration. Its dual role in physiological processes (e.g., wound healing, embryogenesis) and pathological conditions (e.g., tumor angiogenesis, retinopathy) makes it a critical therapeutic and research target.
Recombinant VEGF165 is produced using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications, such as glycosylation, which are essential for bioactivity. Purification techniques like affinity chromatography yield high-purity proteins validated via SDS-PAGE, ELISA, and functional assays (e.g., endothelial cell proliferation tests). Quality control ensures batch consistency for research and clinical applications.
In research, recombinant VEGF165 is widely used to study angiogenesis mechanisms, screen anti-angiogenic drugs, and model vascular diseases. Clinically, it explores therapeutic angiogenesis for ischemic disorders (myocardial infarction, peripheral artery disease) and serves as a reference in cancer studies to evaluate VEGF-targeting therapies (e.g., bevacizumab). Additionally, it supports tissue engineering by stimulating vascularization in regenerative medicine. Its versatility underscores its importance in both basic science and translational medicine.
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