首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔血管内皮细胞生长因子(VEGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VEGF含量。VEGF即血管内皮生长因子，在肿瘤血管生成、伤口愈合等生理过程中起关键作用。其背景在于它能促进内皮细胞增殖、迁移和血管生成。研究机制方面，主要是与受体结合，激活下游信号通路，诱导血管生成，是肿瘤治疗等领域的重要靶点。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于科研场景下探究血管生成相关机制、评估疾病模型中VEGF的动态变化，或用于药物研发中靶向分子效应的体外检测。可为心血管疾病、肿瘤微环境等领域的基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：VEGF
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 91-106 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 97 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.5382.5402.5392.403502.0122.0222.0171.881251.1371.1301.1340.99812.50.6020.6510.6270.4916.250.3750.3780.3770.2413.120.2790.2850.2820.1461.560.1680.1760.1720.03600.1310.1410.136 | ||||||
货期：3-5 working days
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