| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BASP1 |
| Uniprot No | P80723 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGGKLSK KKKGYNVNDE KAKEKDKKAE GAATEEEGTP KESEPQAAAE PAEAKEGKEK PDQDAEGKAE EKEGEKDAAA AKEEAPKAEP EKTEGAAEAK AEPPKAPEQE QAAPGPAAGG EAPKAAEAAA APAESAAPAA GEEPSKEEGE PKKTEAPAAP AAQETKSDGA PASDSKPGSS EAAPSSKETP AATEAPSSTP KAQGPAASAE EPKPVEAPAA NSDQTVTVKE |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BASP1重组蛋白的3-4条参考文献示例(注：文献信息为模拟，仅供参考)：
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1. **文献名称**：*"BASP1 inhibits Wnt/β-catenin signaling via direct interaction with β-catenin in breast cancer"*
**作者**：Smith A, et al.
**摘要**：该研究利用重组人BASP1蛋白，证明其通过直接结合β-catenin抑制Wnt信号通路，从而抑制乳腺癌细胞的迁移和侵袭，揭示了BASP1作为肿瘤抑制因子的潜在机制。
2. **文献名称**：*"Recombinant BASP1 induces neuronal apoptosis through caspase-3 activation"*
**作者**：Johnson R, et al.
**摘要**：研究通过体外表达重组BASP1蛋白，发现其可激活caspase-3依赖性凋亡通路，提示BASP1在神经元程序性死亡中的关键作用，为神经退行性疾病研究提供新靶点。
3. **文献名称**：*"Phosphorylation-dependent membrane association of BASP1: Insights from recombinant protein studies"*
**作者**：Mosevitsky MI, et al.
**摘要**：通过重组BASP1蛋白的磷酸化实验，揭示其膜结合能力受特定激酶调控，并解析了N端结构域在细胞膜定位中的功能，为BASP1的亚细胞动态分布提供分子机制。
4. **文献名称**：*"BASP1 binds to MYST1 and represses histone acetylation in gene regulation"*
**作者**：Lee S, et al.
**摘要**：利用重组BASP1蛋白进行互作实验，发现其与组蛋白乙酰转移酶MYST1结合，抑制特定基因启动子的组蛋白乙酰化水平，阐明BASP1在表观遗传调控中的直接作用。
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**备注**：以上文献为示例性内容，实际引用时需核实真实出版物信息及原文内容。
**Background of BASP1 Recombinant Protein**
BASP1 (Brain Acid-Soluble Protein 1), also known as CAP-23 or NAP-22. is a conserved, multifunctional protein initially identified in neuronal tissues. It is characterized by its high solubility in acidic conditions and distinct structural features, including an N-terminal myristoylation site and a largely unstructured C-terminal region. BASP1 is involved in diverse cellular processes, such as membrane dynamics, cytoskeletal remodeling, and transcriptional regulation.
Functionally, BASP1 plays dual roles in cellular physiology and pathology. In neurons, it contributes to synaptic plasticity, axonal regeneration, and neurite outgrowth by interacting with membrane lipids (e.g., cholesterol-rich domains) and modulating actin cytoskeleton dynamics. Beyond the nervous system, BASP1 acts as a tumor suppressor in cancers like Wilms’ tumor and glioblastoma. It inhibits oncogenic signaling pathways (e.g., Wnt/β-catenin) by binding to transcription factors like WT1 and suppressing pro-metastatic gene expression.
Recombinant BASP1 proteins are engineered using expression systems (e.g., *E. coli* or mammalian cells*) to study its structure-function relationships, post-translational modifications (e.g., myristoylation, phosphorylation), and interactions with cellular partners. These purified proteins serve as critical tools for elucidating BASP1’s role in neurodevelopment, cancer progression, and regenerative medicine. Additionally, recombinant BASP1 has potential therapeutic applications, such as enhancing nerve repair or targeting cancer pathways.
Recent studies also highlight BASP1’s epigenetic regulation and its diagnostic potential as a biomarker in cancer and neurodegenerative disorders. Its versatile nature underscores its importance in both basic research and clinical translation.
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