首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板反应蛋白/凝血酶敏感蛋白1(TSP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的TSP-1含量。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于实验室开展心血管病理机制、肿瘤生物学行为或再生医学相关研究，特别适合需要多类型样本（如同时分析血液样本与组织微环境）的对比性实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TSP-1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TSP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 97 | ||||
| Range % | 94-100 | |||||
| 1:200 | Average % | 89 | ||||
| Range % | 84-92 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of rat TSP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-91 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.4052.4872.4462.2722001.9921.9721.9821.8081001.5831.5981.5911.417501.1311.1681.1500.976250.7480.7280.7380.56412.50.4980.4620.4800.3066.250.3350.3140.3250.15100.1730.1750.174 | ||||||
货期：3-5 working days
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