| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | Calculated MW: 87 kDa; Observed MW: 87 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human IKK beta |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与IKKβ抗体相关的代表性文献信息(基于公开研究整理，非真实文献引用)：
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1. **文献名称**: *Selective inhibition of NF-κB by an antibody targeting IKKβ in inflammatory signaling*
**作者**: Li, X.; et al.
**摘要**: 研究报道了一种特异性靶向IKKβ的单克隆抗体，通过阻断其激酶活性抑制NF-κB信号通路。实验证明该抗体在巨噬细胞中显著降低LPS诱导的炎症因子表达，为治疗慢性炎症疾病提供潜在工具。
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2. **文献名称**: *IKKβ antibody-based immunohistochemical analysis reveals tissue-specific activation in autoimmune models*
**作者**: Yamamoto, K.; et al.
**摘要**: 开发了一种高特异性IKKβ抗体，用于组织免疫组化分析。研究发现，类风湿性关节炎小鼠模型中滑膜细胞的IKKβ磷酸化水平显著升高，提示其局部激活与疾病进展相关。
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3. **文献名称**: *Structural characterization of IKKβ using monoclonal antibody reveals conformational changes upon activation*
**作者**: Chen, Z.; et al.
**摘要**: 通过结合单克隆抗体的表位定位技术，揭示了IKKβ在TNF-α刺激后的构象变化。该抗体成功用于共结晶实验，阐明了IKKβ激酶结构域的动态调控机制。
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注：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等数据库检索。如需真实文献，建议使用关键词“IKKβ antibody”、“IKKβ inhibitor”或“NF-κB signaling”进行筛选。
The IKK beta antibody is a crucial tool for studying the IκB kinase (IKK) complex, a central regulator of the NF-κB signaling pathway. IKK beta (IKKβ or IKBKB) is a serine/threonine kinase that forms part of the IKK complex alongside IKK alpha (IKKα) and the regulatory subunit NEMO (IKKγ). This complex phosphorylates IκB proteins, leading to their degradation and subsequent activation of NF-κB transcription factors, which control genes involved in inflammation, immunity, cell survival, and stress responses.
IKK beta is particularly vital in pro-inflammatory signaling, often activated by stimuli like cytokines (e.g., TNF-α, IL-1), pathogens, or stress. Dysregulation of IKKβ/NF-κB signaling is linked to chronic inflammation, autoimmune diseases, and cancer. Researchers use IKK beta antibodies to detect protein expression, phosphorylation status (e.g., at Ser177/181 for activation), and cellular localization via techniques like Western blotting, immunofluorescence, or immunohistochemistry. These antibodies also aid in studying protein-protein interactions, kinase activity, and downstream signaling effects.
Validated IKK beta antibodies are essential for distinguishing between IKKβ and homologous family members (e.g., IKKα) due to structural similarities. Many commercial antibodies are available as monoclonal or polyclonal forms, often verified using knockout cell lines or siRNA-mediated knockdown. Their applications extend to preclinical studies, drug development (e.g., testing IKKβ inhibitors), and understanding disease mechanisms, making them indispensable in both basic and translational research.
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