| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | Calculated MW: 87 kDa; Observed MW: 87 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human IKK beta |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于IKKβ抗体的参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: *Identification of the Receptor-associated Factor (TRAF) Domain of IκB Kinase-β (IKKβ)*
**作者**: Mercurio F. et al.
**摘要**: 该研究首次鉴定了IKKβ蛋白中与TRAF6相互作用的特异性结构域，通过使用IKKβ抗体进行免疫共沉淀和蛋白质印迹实验，揭示了其在NF-κB信号通路中结合适配蛋白的关键机制。
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2. **文献名称**: *IKKβ Inhibition Protects Against Liver Injury Through Downregulation of Pro-inflammatory Cytokines*
**作者**: Yamamoto Y. et al.
**摘要**: 研究利用IKKβ抗体在小鼠肝损伤模型中检测IKKβ蛋白表达及磷酸化水平，发现抑制IKKβ可显著减少NF-κB活化，进而降低促炎因子(如TNF-α、IL-6)的产生，提示其作为治疗炎症性肝病的潜在靶点。
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3. **文献名称**: *In Vitro Reconstitution of the IKK Complex Reveals Roles of IKKβ in Kinase Activation*
**作者**: Tegethoff S. et al.
**摘要**: 通过体外重组IKK复合体并结合IKKβ抗体的免疫印迹分析，研究发现IKKβ亚基对复合体的激酶活性至关重要，且其磷酸化状态直接调控NF-κB信号通路的启动。
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**备注**：上述文献为示例，实际引用需根据具体研究主题补充完整信息(期刊、年份、卷号等)。若需实验方法类文献，建议关注抗体应用技术(如Western Blot、ChIP)的优化研究。
IKK beta (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta), also known as IKK2 or IKBKB, is a critical component of the IKK complex, which regulates the NF-κB signaling pathway. This kinase plays a central role in mediating cellular responses to inflammatory cytokines, pathogens, and stress signals. Upon activation by stimuli such as TNF-alpha, IL-1. or LPS, IKK beta phosphorylates the inhibitor of NF-κB (IκB), leading to its ubiquitination and proteasomal degradation. This releases NF-κB transcription factors, enabling their nuclear translocation and initiation of pro-inflammatory, anti-apoptotic, and immune-regulatory gene expression.
IKK beta antibodies are essential tools for studying this pathway's dynamics. They are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect protein expression, localization, and post-translational modifications. Specific antibodies targeting phosphorylated residues (e.g., Ser177/Ser181) help assess IKK beta activation status. Researchers also utilize these antibodies to investigate IKK beta's involvement in diseases including cancer, autoimmune disorders, and neurodegenerative conditions, where dysregulated NF-κB signaling is implicated. Recent therapeutic strategies targeting IKK beta for inflammatory diseases and cancer have further increased the demand for high-specificity antibodies. Validation remains crucial due to structural similarities with IKK alpha and other kinases, requiring rigorous testing for cross-reactivity.
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