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Mouse Thymic stromal lymphopoietin(TSLP) ELISA kit

  • 中文名： 小鼠胸腺基质淋巴细胞生成素(TSLP)酶联免疫试剂盒
货号: IPD-EL025141MO
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠胸腺基质淋巴细胞生成素(TSLP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TSLP含量。TSLP即胸腺基质淋巴细胞生成素，是一种上皮细胞来源的细胞因子。它在免疫调节中起重要作用，尤其在固有免疫和适应性免疫的连接环节发挥关键功能。研究表明其与多种炎症性疾病如哮喘、特应性皮炎等发病机制相关，是开发相关疾病治疗药物的潜在靶点。试剂盒检测范围为15.6 pg/ml-1000 pg/ml，广泛应用于基础科研领域，适用于构建过敏性炎症、呼吸道疾病等小鼠模型中TSLP的动态变化分析，也可用于评估药物干预或基因修饰对TSLP表达的影响，为免疫调节机制及疾病机制研究提供可靠工具。操作流程严格遵循标准化ELISA实验步骤，兼容常规酶标仪检测，满足实验室对复杂样本的高通量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Tslp ELISA Kit; Thymic stromal lymphopoietin ELISA Kit; Thymic stroma-derived lymphopoietin ELISA Kit
  • 缩写：
    TSLP
  • Uniprot No.：
    Q9JIE6
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/ml-1000 pg/ml
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TSLP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    92


    Range %

    85-99


    1:2

    Average %

    85


    Range %

    81-90


    1:4

    Average %

    94


    Range %

    87-101


    1:8

    Average %

    93


    Range %

    86-100


  • 回收率：

    The recovery of mouse TSLP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    89

    83-95


    EDTA plasma (n=4)

    95

    91-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    3.103

    2.934

    3.018

    2.894


    500

    2.462

    2.367

    2.414

    2.290


    250

    1.560

    1.628

    1.594

    1.470


    125

    0.834

    0.829

    0.832

    0.707


    62.5

    0.515

    0.521

    0.518

    0.393


    31.2

    0.343

    0.353

    0.348

    0.223


    15.6

    0.219

    0.224

    0.222

    0.097


    0

    0.124

    0.125

    0.125




  • 货期：
    3-5 working days

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