| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B |
| Entrez GeneID | 1147 |
| WB Predicted band size | Calculated MW: 85 kDa; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human IKK alpha |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与IKKα抗体相关的参考文献摘要示例(文献信息为虚构，供参考)：
1. **"IKKα regulates epidermal differentiation through NF-κB and TGF-β signaling pathways"**
*Authors: Li Q, Lu Q, Estepa G, Verma IM*
摘要：该研究利用特异性IKKα抗体，通过免疫组化和Western blot分析，揭示了IKKα在小鼠表皮分化中的作用，发现其缺失导致角质化异常，并影响TGF-β通路调控。
2. **"Selective inhibition of IKKα attenuates prostate cancer progression in vivo"**
*Authors: Luo JL, Kamata H, Karin M*
摘要：研究开发了靶向IKKα的单克隆抗体，验证其在抑制NF-κB非经典通路中的特异性，并通过体内实验证明阻断IKKα可减少前列腺癌转移，抗体用于组织样本的磷酸化蛋白检测。
3. **"IKKα antibody-based profiling reveals subcellular localization dynamics in immune cells"**
*Authors: Greten FR, Arkan MC, Bollrath J*
摘要：通过高特异性IKKα抗体进行共聚焦显微镜分析，发现炎症刺激下IKKα从细胞质向核周区域转位，提示其参与TLR信号传导的时空调控机制。
*注：以上文献为示例模板，实际文献需通过PubMed/Google Scholar检索关键词(如"IKKα antibody"、"IKK alpha immunoassay")获取。真实文献可能侧重抗体开发(如：品牌说明书)或特定功能研究中的抗体应用。*
The IKK alpha (IκB kinase alpha) antibody is a crucial tool in studying the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathway, which regulates inflammation, immune responses, cell proliferation, and apoptosis. IKK alpha, encoded by the *IKBKA* gene, is a serine/threonine kinase and a core component of the IKK complex, alongside IKK beta (IKKβ) and NEMO (NF-κB essential modulator). While IKK beta primarily drives canonical NF-κB activation by phosphorylating IκB inhibitors, IKK alpha plays a dual role: it contributes to non-canonical NF-κB signaling and regulates developmental processes, such as epidermal differentiation and lymphoid organogenesis.
IKK alpha antibodies are widely used in research to detect protein expression, phosphorylation status (e.g., at Ser176/180), and subcellular localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate IKK alpha’s context-dependent functions, including its role in cancer progression, immune disorders, and skin diseases. Commercially available antibodies target specific epitopes or post-translational modifications, enabling precise mechanistic studies. Dysregulation of IKK alpha is linked to chronic inflammation, autoimmune diseases, and tumorigenesis, making it a potential therapeutic target. Researchers rely on high-specificity IKK alpha antibodies to dissect its interactions with signaling partners, validate genetic models (e.g., knockout mice), and assess drug efficacy in preclinical studies.
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