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Human thymic stromal lymphopoietin,TSLP ELISA Kit

  • 中文名： 人胸腺基质淋巴细胞生成素(TSLP)ELISA Kit
货号: IPD-E09316h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人胸腺基质淋巴细胞生成素(TSLP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的TSLP含量。TSLP即胸腺基质淋巴细胞生成素，它是一种上皮细胞因子，在免疫系统中扮演重要角色。其能激活树突状细胞，调节T细胞分化，参与多种免疫反应。目前研究聚焦于它与哮喘、特应性皮炎等炎症性疾病的关联，有望成为相关疾病治疗新靶点。试剂盒检测范围为0.312 ng/ml-20 ng/ml，适用于探索过敏反应机制、评估药物干预效果或比较不同疾病模型中的TSLP动态变化。研究人员可通过该工具分析呼吸道或皮肤组织匀浆中TSLP的表达差异，研究免疫细胞激活过程中血浆/血清生物标志物的波动规律，或验证基因编辑/抑制剂处理对靶蛋白分泌的影响，为免疫调节研究和炎症性疾病机制解析提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Thymic stromal lymphopoietin ELISA Kit; Thymic stromal lymphopoietin protein TSLP ELISA Kit; Tslp ELISA Kit; TSLP protein ELISA Kit; TSLP_HUMAN ELISA Kit
  • 缩写：
    TSLP
  • Uniprot No.：
    Q969D9
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.312 ng/ml-20 ng/ml
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human TSLP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    84-97


    1:2

    Average %

    92


    Range %

    86-99


    1:4

    Average %

    91


    Range %

    84-98


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human TSLP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    92

    86-97


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    3.002

    2.934

    2.968

    2.786


    10

    1.935

    1.867

    1.901

    1.719


    5

    1.163

    1.128

    1.146

    0.964


    2.5

    0.734

    0.729

    0.732

    0.550


    1.25

    0.406

    0.421

    0.413

    0.231


    0.625

    0.306

    0.293

    0.299

    0.118


    0.312

    0.246

    0.254

    0.250

    0.068


    0

    0.179

    0.185

    0.182



  • 本试剂盒所含材料：

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    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human TSLP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled TSLP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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