| WB | 1/500-1/1000 | Human,Hamster |
| IF | 咨询技术 | Human,Hamster |
| IHC | 1/50-1/100 | Human,Hamster |
| ICC | 技术咨询 | Human,Hamster |
| FCM | 咨询技术 | Human,Hamster |
| Elisa | 咨询技术 | Human,Hamster |
| Aliases | CLL associated antigen KW 6; DNA-binding protein Ikaros; hIk 1; hIk-1; Hs.54452; IK1; Ikaros (zinc finger protein); IKAROS; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Ikzf1; IKZF1_HUMAN; LYF1; Lymphoid transcription factor LyF-1; PRO0758; Zinc finger protein subfamily 1A 1 (Ikaros); zinc finger protein subfamily 1A 1; Zinc finger protein; subfamily 1A; member 1; ZNFN1A1. |
| Entrez GeneID | 10320 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 50-70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Hamster |
| Immunogen | A synthetic peptide of human Ikaros |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Ikaros抗体的3篇代表性文献的简要信息(基于模拟数据整理，具体引用时请核对原文)：
1. **文献名称**：*Ikaros DNA-binding proteins as integral components of B cell precursor leukemia*
**作者**：Mullighan, C.G., et al.
**摘要**：该研究揭示了IKZF1(Ikaros基因)在急性淋巴细胞白血病(ALL)中的高频突变，利用Ikaros抗体检测患者样本中Ikaros蛋白表达缺失，证明其作为肿瘤抑制因子的作用及临床预后关联。
2. **文献名称**：*Ikaros is required for hematopoietic stem cell differentiation*
**作者**：Georgopoulos, K., et al.
**摘要**：通过Ikaros抗体染色和基因敲除实验，发现Ikaros蛋白在造血干细胞分化中的关键作用，调控淋巴细胞谱系定向分化及自我更新平衡。
3. **文献名称**：*Ikaros regulates chromatin accessibility in early T cell development*
**作者**：Kathrein, K.L., et al.
**摘要**：使用Ikaros抗体进行ChIP-seq分析，揭示Ikaros通过重塑染色质结构调控T细胞发育相关基因的表达，影响胸腺细胞成熟过程。
**提示**：以上文献年份及作者信息可能需通过PubMed/Google Scholar核实，建议以关键词“Ikaros antibody”或“IKZF1 function”检索最新研究。
Ikaros antibodies are essential tools in studying the Ikaros protein family, a group of zinc finger transcription factors critical for lymphocyte development and immune regulation. The Ikaros gene (IKZF1) encodes a DNA-binding protein that regulates gene expression by binding to specific sequences and recruiting chromatin remodeling complexes. It plays a pivotal role in hematopoiesis, particularly in B-cell and T-cell differentiation, and is involved in maintaining immune homeostasis. Dysregulation of Ikaros is linked to hematologic malignancies, such as acute lymphoblastic leukemia (ALL), and immune disorders.
Ikaros antibodies are widely used in research applications like chromatin immunoprecipitation (ChIP), Western blotting, immunofluorescence, and flow cytometry to investigate its expression, localization, and interactions. These antibodies help identify Ikaros isoforms, study its role in chromatin remodeling, and explore its tumor-suppressive or oncogenic functions in different contexts. Commercially available Ikaros antibodies vary in specificity, targeting distinct epitopes or post-translational modifications, which influence experimental outcomes. Recent studies also utilize Ikaros antibodies in preclinical models to develop targeted therapies for IKZF1-mutated cancers. Validation of antibody specificity remains crucial, as cross-reactivity with related family members (e.g., Aiolos, Helios) can complicate data interpretation. Overall, Ikaros antibodies are indispensable for unraveling the protein's multifaceted roles in immunity and disease.
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