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产品描述：兔子促甲状腺素(TSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TSH含量。促甲状腺激素（TSH）是腺垂体分泌的促进甲状腺生长和机能的激素。其分泌受下丘脑分泌的促甲状腺激素释放激素调节。研究机制方面，TSH 与甲状腺滤泡上皮细胞膜上受体结合，刺激甲状腺激素合成与释放，对维持甲状腺的正常功能至关重要。试剂盒检测范围为0.5 μIU/mL-20 μIU/mL，适用于科研场景中甲状腺相关疾病的动物模型研究、药物干预对激素分泌的影响分析或基础内分泌机制探索；为实验动物TSH水平监测提供可靠工具；特别推荐用于小动物内分泌系统研究的纵向观察或跨组学关联分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TSH
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.5 μIU/mL-20 μIU/mL
灵敏度：0.25 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of rabbit TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average202.8312.8362.834102.0522.0792.06651.1781.1431.1612.50.6590.6390.64910.2600.2690.2650.50.1240.1240.124 | ||||||
货期：3-5 working days
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