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产品描述：猪促甲状腺素(TSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TSH含量。TSH即促甲状腺激素，是腺垂体分泌的促进甲状腺生长和机能的激素。其主要作用是调节甲状腺细胞的增殖和甲状腺血液供应，调控甲状腺细胞合成与释放甲状腺激素。在科研中，常围绕其与甲状腺疾病关系等机制展开研究，以助力疾病诊断与治疗。试剂盒检测范围为0.6 μIU/mL-24 μIU/mL，适用于动物生理学研究、药物开发中的甲状腺功能评估、畜牧养殖健康监测等场景，例如探究环境应激对甲状腺轴的影响、饲料添加剂或激素干预的效果分析等本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
缩写：TSH
Uniprot No.：P01224
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：0.6 μIU/mL-24 μIU/mL
灵敏度：0.3 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of pig TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 102 | 98-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2Average242.3542.2192.287121.2521.2151.23460.6350.6480.64230.3270.3100.3191.20.1550.1520.1540.60.0790.0780.079 | ||||||
货期：3-5 working days
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