WB | 1/500-1/1000 | Human,Mouse,Rat |
IF | 1/20 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | insulin-like growth factor 2 mRNA binding protein 1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1 |
Entrez GeneID | 10642 |
WB Predicted band size | Calculated MW: 63 kDa; Observed MW: 63 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse |
Immunogen | Recombinant protein of human IGF2BP1 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于IGF2BP1抗体的参考文献摘要示例:
1. **"IGF2BP1 as a novel biomarker in colorectal cancer progression"**
*Author: Zhang Y, et al.*
**摘要**: 该研究通过免疫组化分析IGF2BP1在结直肠癌组织中的高表达,揭示其与患者预后不良的相关性,并利用特异性抗体证实其通过稳定致癌mRNA促进肿瘤转移。
2. **"The role of IGF2BP1 in regulating mRNA stability and cancer metabolism"**
*Author: Müller S, et al.*
**摘要**: 文章利用IGF2BP1抗体进行RNA免疫沉淀测序(RIP-seq),发现其靶向结合代谢相关基因转录本,调控肿瘤细胞糖酵解过程,影响乳腺癌细胞增殖。
3. **"IGF2BP1 antibody-based detection in pancreatic ductal adenocarcinoma"**
*Author: Wang H, et al.*
**摘要**: 研究开发了一种高灵敏度的IGF2BP1单克隆抗体,验证其在胰腺癌组织中的特异性表达,证明其可作为早期诊断的生物标志物,并与化疗耐药性相关。
4. **"IGF2BP1 interacts with lncRNAs to promote hepatocellular carcinoma invasion"**
*Author: Chen L, et al.*
**摘要**: 通过免疫共沉淀(Co-IP)结合IGF2BP1抗体,发现其与长链非编码RNA(lncRNA-ATB)相互作用,激活Wnt/β-catenin通路,驱动肝癌细胞侵袭。
注:以上为示例性内容,实际文献需通过学术数据库检索确认。
The IGF2BP1 (Insulin-like Growth Factor 2 mRNA-Binding Protein 1) antibody is a tool used to detect and study the IGF2BP1 protein, a member of the conserved RNA-binding protein family involved in post-transcriptional gene regulation. IGF2BP1. also known as IMP1 or ZBP1. contains KH domains that enable binding to target mRNAs, influencing their stability, localization, and translation. It plays critical roles in embryonic development, cell polarization, and tumorigenesis by regulating oncogenic mRNAs like MYC, IGF2. and β-catenin.
In cancer research, IGF2BP1 is recognized as an oncofetal protein, often overexpressed in malignancies such as glioblastoma, lung, ovarian, and colorectal cancers, where it promotes proliferation, invasion, and chemoresistance. Its expression correlates with poor prognosis, making it a potential biomarker. The IGF2BP1 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression patterns, subcellular localization, and interactions in both physiological and pathological contexts. Validation of antibody specificity is crucial, as cross-reactivity with homologous family members (IGF2BP2/3) can occur. Studies utilizing this antibody contribute to understanding post-transcriptional regulatory mechanisms in development, stem cell biology, and cancer progression.
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