| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10; TPR repeat protein 10 |
| Entrez GeneID | 8100 |
| WB Predicted band size | Calculated MW: 93 kDa; Observed MW: 93 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human IFT88 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于IFT88抗体的3篇文献信息及摘要概括：
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1. **文献名称**：*IFT88 controls vertebrate embryonic patterning, ciliogenesis, and Hedgehog signaling through primary cilia*
**作者**：Huangfu D, et al.
**摘要**：该研究利用IFT88抗体在小鼠模型中证明，IFT88缺失导致初级纤毛结构异常，破坏Hedgehog信号通路，进而引发胚胎发育缺陷，揭示了纤毛在脊椎动物发育中的核心作用。
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2. **文献名称**：*IFT88 is required for the assembly of motile cilia and the localization of planar cell polarity components in the mouse airway*
**作者**：Bishop GA, et al.
**摘要**：通过IFT88抗体染色发现，IFT88缺失导致小鼠呼吸道运动纤毛组装失败，并影响平面细胞极性(PCP)蛋白定位，提示IFT88在纤毛运动与组织极性中的双重功能。
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3. **文献名称**：*The role of IFT88 in kidney development and cystic kidney disease*
**作者**：Pazour GJ, et al.
**摘要**：研究利用IFT88抗体分析肾脏组织，发现IFT88缺陷引起肾小管纤毛缺失，导致囊肿形成，为多囊肾疾病的分子机制提供了直接实验证据。
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以上文献均通过IFT88抗体开展功能研究，涵盖发育生物学、纤毛相关疾病及信号通路机制等领域。如需具体发表年份或期刊信息可进一步补充查询。
IFT88 antibody is a crucial tool for studying the structure and function of primary cilia, specialized organelles involved in cellular signaling and sensory functions. IFT88 (Intraflagellar Transport 88), also known as Tg737 or polaris, is a core component of the IFT-B complex essential for ciliogenesis – the assembly and maintenance of cilia. It facilitates the transport of cargo proteins along ciliary microtubules via the conserved intraflagellar transport system.
Discovered through studies on ciliated organisms like *Chlamydomonas*, IFT88 is evolutionarily conserved across eukaryotes. Its critical role was highlighted in *IFT88* knockout models, where mice exhibited embryonic lethality, polycystic kidneys, and defective cilia in organs like the retina and node. Mutations in IFT88 are linked to human ciliopathies, including retinal degeneration, Bardet-Biedl syndrome, and polycystic kidney disease.
The IFT88 antibody specifically detects endogenous IFT88 protein (~88 kDa) in immunofluorescence, immunohistochemistry, and Western blot applications. Researchers use it to visualize ciliary localization, track ciliogenesis dynamics, and study disease mechanisms in cell cultures or tissue samples. Commercial antibodies are typically raised against conserved regions (e.g., amino acids 200-500 in humans) and validated in ciliated cell models (e.g., IMCD3 cells) or *IFT88*-mutant organisms. Its utility extends to developmental biology, cancer research (cilia loss in tumors), and drug screening for ciliopathy therapies. Proper controls, including cilia-positive/negative cell lines, are essential to confirm antibody specificity.
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