| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UXT |
| Uniprot No | Q9UBK9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-157aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MATPPKRRAV EATGEKVLRY ETFISDVLQR DLRKVLDHRD KVYEQLAKYL QLRNVIERLQ EAKHSELYMQ VDLGCNFFVD TVVPDTSRIY VALGYGFFLE LTLAEALKFI DRKSSLLTEL SNSLTKDSMN IKAHIHMLLE GLRELQGLQN FPEKPHH |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UXT重组蛋白的3篇文献摘要示例(注：文献年份及作者为虚构示例，实际文献需根据具体数据库检索验证)：
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1. **文献名称**: *UXT Acts as a Coactivator for Androgen Receptor Signaling through Interactions with ARA54*
**作者**: Meng, X. et al. (2010)
**摘要**: 该研究通过重组UXT蛋白的体外表达，证明其作为雄激素受体(AR)的共激活子，与ARA54相互作用增强AR转录活性，揭示了UXT在激素信号通路中的分子伴侣功能。
2. **文献名称**: *Structural Insights into UXT as a Molecular Chaperone in the NF-κB Signaling Pathway*
**作者**: Zhang, Y. et al. (2015)
**摘要**: 利用重组UXT蛋白的晶体结构解析，发现UXT通过稳定IκB激酶复合物(IKK)构象促进NF-κB活化，阐明其在先天免疫应答中的结构基础。
3. **文献名称**: *UXT Recombinant Protein Attenuates Oxidative Stress by Enhancing Nrf2 Nuclear Translocation*
**作者**: Li, H. et al. (2020)
**摘要**: 研究显示，外源性重组UXT蛋白可通过结合Keap1促进Nrf2入核，激活抗氧化基因表达，为UXT在氧化应激相关疾病中的治疗潜力提供依据。
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建议通过PubMed或Web of Science以“UXT recombinant”、“UXT chaperone”等关键词检索真实文献。实际研究中，UXT多与转录调控、应激反应及癌症通路相关。
UXT (Ubiquitously Expressed Transcript), also known as ART-27 or STAP1. is a highly conserved protein encoded by the UXT gene located on human chromosome Xq13.1. Initially identified as a ubiquitously expressed transcript in the early 2000s, UXT belongs to the family of small chaperone-like proteins. Structurally, it contains tandem tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions. UXT lacks intrinsic enzymatic activity but functions as a co-activator or adaptor molecule in transcriptional regulation, particularly interacting with nuclear hormone receptors (e.g., androgen receptor, AR) and the NF-κB signaling pathway. It facilitates the assembly of transcriptional complexes by stabilizing protein interactions.
Recombinant UXT proteins are engineered through molecular cloning, typically expressed in bacterial (e.g., E. coli) or mammalian systems to study its biochemical properties and interactions. The recombinant form retains critical domains for binding partners like PML (promyelocytic leukemia protein) and components of the centrosome, linking it to cellular processes such as stress response, cell cycle regulation, and apoptosis. Research highlights its dual role: as a tumor suppressor in certain cancers (e.g., prostate cancer via AR modulation) and as a potential oncogene in others (e.g., hepatocellular carcinoma via NF-κB activation). Its involvement in ciliogenesis and centrosome dynamics further connects it to developmental disorders and ciliopathies. Current studies utilize recombinant UXT to dissect its structural motifs, post-translational modifications, and therapeutic targeting potential. The protein’s small size (~18 kDa) and stability make it a practical model for structural biology and interaction network mapping.
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