| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HIP116; HIP116A; HLTF 1; Hltf; HLTF1; p113; RNF80; SMARC A3; SMARCA 3; SMARCA3; SNF2L3; ZBU1 |
| Entrez GeneID | 6596 |
| WB Predicted band size | Calculated MW: 114 kDa; Observed MW: 114 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human HLTF |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于HLTF抗体的3篇参考文献示例(文献名称、作者及摘要内容概括)：
1. **"HLTF promotes DNA damage tolerance and regulates transcription in human cells"**
- **作者**: Motegi A, et al.
- **摘要**: 研究揭示了HLTF蛋白在DNA损伤修复中的关键作用，利用特异性HLTF抗体验证其与PCNA的相互作用，表明HLTF通过泛素化修饰调控复制后修复机制，维持基因组稳定性。
2. **"Antibody-based profiling of HLTF expression in colorectal cancer"**
- **作者**: Karczmarski J, et al.
- **摘要**: 通过开发高特异性HLTF单克隆抗体，分析了结直肠癌组织中HLTF的异常表达模式，发现其低表达与患者预后不良显著相关，提示HLTF可能作为抑癌分子参与肿瘤发生。
3. **"Structural and functional characterization of the HLTF PARP-like domain using targeted antibodies"**
- **作者**: Burrows AC, et al.
- **摘要**: 研究利用定制HLTF抗体解析其PARP样结构域的功能，证明该结构域在DNA损伤修复中的酶活调控机制，并验证抗体在染色质免疫沉淀(ChIP)中的应用效果。
4. **"HLTF depletion disrupts homologous recombination via impaired chromatin remodeling"**
- **作者**: Kim J, et al.
- **摘要**: 通过RNA干扰和HLTF抗体验证实验，证实HLTF通过SWI/SNF复合物介导的染色质重塑促进同源重组修复，其缺失导致DNA修复缺陷和细胞放射敏感性增加。
(注：上述文献信息为示例，实际引用时请核对真实文献。)
**Background of HLTF Antibody**
HLTF (HepA-related transcription factor), also known as HIP116 or SMARCA3. is a member of the SWI/SNF family of chromatin-remodeling proteins. It plays a critical role in DNA damage repair, epigenetic regulation, and maintenance of genomic stability. HLTF is involved in the error-free post-replication repair pathway, particularly in resolving stalled replication forks during replication stress. It facilitates template switching or translesion synthesis (TLS) to bypass DNA lesions, thereby preventing mutagenesis.
HLTF has been implicated in cancer biology, where its epigenetic silencing (via promoter hypermethylation) is observed in various malignancies, including colorectal, gastric, and ovarian cancers. This silencing correlates with microsatellite instability, poor prognosis, and resistance to chemotherapy. HLTF antibodies are essential tools for detecting its expression in research and diagnostics. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to study HLTF's role in DNA repair mechanisms, tumor suppression, and interactions with other proteins (e.g., BRCA1. PCNA).
Structurally, HLTF contains a HIRAN domain (for DNA binding), a SWI/SNF helicase domain (ATP-dependent chromatin remodeling), and a RING finger domain (ubiquitin ligase activity). Antibodies targeting specific domains help dissect its multifunctional roles. Dysregulation of HLTF highlights its importance as a potential biomarker and therapeutic target in cancer and other genomic instability-related disorders.
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