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产品描述：人促甲状腺素释放激素(TRH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TRH含量。TRH即促甲状腺激素释放激素，它由下丘脑分泌，可刺激腺垂体分泌促甲状腺激素。其背景在于它是调节甲状腺功能的重要神经内分泌激素。研究机制主要围绕其对甲状腺轴的调控，及在神经系统中对神经递质释放、神经细胞生长分化等方面作用展开。试剂盒检测范围为2.5 pg/mL-100 pg/mL，本试剂盒适用于科研领域中对TRH表达水平的精准定量分析，可应用于神经内分泌调节机制研究、甲状腺功能相关疾病模型建立、内分泌系统与代谢关联性探索等方向。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TRH ELISA Kit; Pro-thyrotropin-releasing hormone ELISA Kit; Pro-TRH ELISA Kit; Prothyroliberin) [Cleaved into: Thyrotropin-releasing hormone ELISA Kit; TRH ELISA Kit; Protirelin ELISA Kit; TSH-releasing factor ELISA Kit; Thyroliberin ELISA Kit; Thyrotropin-releasing factor ELISA Kit; TRF)] ELISA Kit
缩写：TRH
Uniprot No.：P20396
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：2.5 pg/mL-100 pg/mL
灵敏度：1.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 81-92 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 85-105 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 92-102 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of human TRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 89-98 | |||||
| EDTA plasma (n=4) | 97 | 91-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average1002.2322.2522.242501.1961.1561.176250.6350.6480.64212.50.3570.3620.36050.1460.1510.1492.50.0750.0790.077 | |||||||
货期：3-5 working days
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