| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP46 |
| Uniprot No | P62068 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-366aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMTVRNIA SICNMGTNAS ALEKDIGPEQ FPINEHYFGL VNFGNTCYCN SVLQALYFCR PFRENVLAYK AQQKKKENLL TCLADLFHSI ATQKKKVGVI PPKKFISRLR KENDLFDNYM QQDAHEFLNY LLNTIADILQ EEKKQEKQNG KLKNGNMNEP AENNKPELTW VHEIFQGTLT NETRCLNCET VSSKDEDFLD LSVDVEQNTS ITHCLRDFSN TETLCSEQKY YCETCCSKQE AQKRMRVKKL PMILALHLKR FKYMEQLHRY TKLSYRVVFP LELRLFNTSS DAVNLDRMYD LVAVVVHCGS GPNRGHYITI VKSHGFWLLF DDDIVEKIDA QAIEEFYGLT SDISKNSESG YILFYQSRE |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于USP46重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*USP46 regulates GABA receptor stability through deubiquitination in vitro*
**作者**：Hussain, S. et al.
**摘要**：本研究利用重组USP46蛋白在体外验证其去泛素化酶活性，发现其通过特异性去除GABAA受体γ2亚基的泛素链，增强受体稳定性，暗示其在神经信号调控中的作用。实验采用大肠杆菌表达纯化的USP46重组蛋白进行酶活性和底物结合分析。
2. **文献名称**：*Structural and functional analysis of USP46 reveals a role in β-catenin signaling*
**作者**：Kim, J.H. et al.
**摘要**：通过重组USP46蛋白的晶体结构解析，揭示了其催化结构域的关键氨基酸残基如何介导与β-catenin的相互作用。功能实验表明，USP46重组蛋白在结直肠癌细胞中抑制Wnt/β-catenin通路活性，提示其作为肿瘤抑制因子的潜力。
3. **文献名称**：*USP46 interacts with histone modifiers and regulates chromatin remodeling*
**作者**：Liang, Y. et al.
**摘要**：利用重组USP46蛋白进行体外互作筛选，发现其与组蛋白去乙酰化酶HDAC1/2结合，并通过去泛素化调控HDAC复合物的稳定性。研究揭示了USP46在表观遗传调控中的新机制，重组蛋白实验证实其直接酶活性和蛋白结合能力。
(注：以上文献为虚拟示例，若需真实文献，建议通过PubMed或Google Scholar以“USP46 recombinant protein”等关键词检索近年研究。)
The USP46 (Ubiquitin-Specific Protease 46) recombinant protein is a engineered form of the deubiquitinating enzyme USP46. which plays a critical role in regulating protein stability and cellular processes through the removal of ubiquitin chains from target substrates. USP46 belongs to the ubiquitin-specific protease family, characterized by a conserved catalytic domain that hydrolyzes isopeptide bonds between ubiquitin molecules and substrate proteins. This enzyme is implicated in diverse biological functions, including neuronal development, synaptic plasticity, and circadian rhythm regulation. Studies link USP46 dysfunction to neurological disorders, psychiatric conditions (e.g., depression, anxiety), and cancer progression, highlighting its therapeutic potential.
Recombinant USP46 protein is typically produced in heterologous expression systems (e.g., *E. coli*, mammalian cells) to ensure high purity and activity for *in vitro* studies. Its structure often includes conserved domains like the USP catalytic core, which harbors critical cysteine and histidine residues for enzymatic activity. Researchers utilize this protein to investigate substrate specificity, enzymatic kinetics, and regulatory mechanisms. For example, USP46 is known to interact with β-catenin and the GABAA receptor, modulating Wnt signaling and neurotransmitter receptor trafficking, respectively. Additionally, USP46 recombinant protein aids in drug discovery, enabling high-throughput screening for inhibitors or activators targeting deubiquitination pathways. Its application extends to disease models, where manipulating USP46 activity helps clarify its role in cellular homeostasis and pathology.
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