| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAP18 |
| Uniprot No | Q8IXM2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMTSASTK VGEIFSAAGA AFTKLGELTM QLHPVADSSP AGAKWTETEI EMLRAAVKRF GDDLNHISCV IKERTVAQIK ATVKRKVYED SGIPLPAESP KKGPKKVASG VLSPPPAAPP PSSSSVPEAG GPPIKKQKAD VTLSALNDSD ANSDVVDIEG LGETPPAKKL NFDQA |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BAP18重组蛋白的3篇文献示例(注：文献为模拟示例，实际需根据真实研究补充)：
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1. **文献名称**：*BAP18 Facilitates Chromatin Accessibility and Modulates Transcriptional Regulation in Stem Cells*
**作者**：Smith A, et al.
**摘要**：研究通过重组BAP18蛋白体外实验，揭示了其通过增强染色质可及性调控胚胎干细胞分化，证实其与组蛋白修饰复合物相互作用，影响关键基因转录活性。
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2. **文献名称**：*Recombinant BAP18 Protein Expression and Its Role in DNA Damage Response*
**作者**：Wang X, et al.
**摘要**：报道了BAP18重组蛋白在大肠杆菌中的高效表达与纯化，并证明其在细胞中参与DNA损伤修复通路，通过体外结合实验验证其与ATM激酶的相互作用。
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3. **文献名称**：*Structural and Functional Analysis of BAP18 in Epigenetic Regulation*
**作者**：Lee S, et al.
**摘要**：利用重组BAP18蛋白进行晶体结构解析，发现其N端结构域对组蛋白H3K4甲基化修饰具有调控作用，为理解其表观遗传功能提供结构基础。
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**注意**：以上内容为示例，实际文献需通过学术数据库(如PubMed、Web of Science)以关键词“BAP18 recombinant”或“BAP18 protein function”检索。若研究较少，可扩展至相关复合物(如BAP18与BPTF的协同作用)或疾病模型中的功能研究。
**Background of BAP18 Recombinant Protein**
BAP18 (BRCA1-associated protein 18) is a chromatin-associated protein implicated in transcriptional regulation and epigenetic modification. It interacts with BRCA1. a tumor suppressor protein critical for DNA repair and genomic stability, linking it to pathways involved in cancer biology. BAP18 is a component of histone acetyltransferase (HAT) complexes, such as the ATAC or STAGA complexes, which mediate histone acetylation—a key epigenetic mark that relaxes chromatin structure and promotes gene activation. This association underscores its role in chromatin remodeling and transcriptional coactivation.
Studies suggest BAP18 regulates the expression of genes involved in cell proliferation, differentiation, and stress responses. Its interaction with BRCA1 highlights its potential involvement in DNA damage repair mechanisms, particularly in homologous recombination. Dysregulation of BAP18 has been observed in cancers, including breast and ovarian cancers, often correlating with BRCA1 mutations.
Recombinant BAP18 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies to dissect its molecular interactions, enzymatic activities, and regulatory roles. Researchers utilize it to explore its binding partners (e.g., histones, transcription factors), its influence on histone acetylation patterns, and its broader impact on chromatin dynamics. Additionally, recombinant BAP18 serves as a tool to investigate its therapeutic potential, either as a biomarker or a target for epigenetic therapies in cancers linked to BRCA1 pathway alterations.
Current research focuses on elucidating BAP18’s structural domains (e.g., conserved PHD fingers) and its crosstalk with signaling pathways, offering insights into epigenetic regulation and cancer progression mechanisms.
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