| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H3F3; H3.3A |
| Entrez GeneID | 3020 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Histone H3.3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"Histone H3.3 mutations drive pediatric glioblastoma through epigenome destabilization"**
- **Authors**: Nacev, B.A., et al.
- **摘要**: 该研究揭示了组蛋白H3.3在儿童胶质母细胞瘤中的关键作用，发现H3.3突变(如K27M和G34R/V)通过破坏染色质修饰和基因表达调控促进肿瘤发生，为靶向表观遗传异常提供了依据。
2. **"Distinct factors control histone variant H3.3 localization at specific genomic regions"**
- **Authors**: Goldberg, A.D., et al.
- **摘要**: 通过ChIP-seq和功能实验，阐明了H3.3在基因启动子、增强子和异染色质区域的差异分布机制，强调其依赖HIRA和DAXX/ATRX复合体的不同组装途径，影响基因活性和染色质稳定性。
3. **"H3.3 maintains genome integrity during mammalian development"**
- **Authors**: Tagami, H., et al.
- **摘要**: 研究利用H3.3敲除模型，发现H3.3在胚胎发育中通过维持端粒和着丝粒区域染色质结构，防止DNA损伤和染色体异常，揭示了其对于基因组稳定性的关键作用。
4. **"Antibody-based detection of histone H3.3 G34W and G34R mutations in giant cell tumor of bone and chondroblastoma"**
- **Authors**: Lewis, P.W., et al.
- **摘要**: 开发了一种特异性识别H3.3 G34W/R突变的抗体，用于骨巨细胞瘤和软骨母细胞瘤的临床诊断，验证了该抗体在区分肿瘤亚型和指导治疗中的实用性。
Histone H3.3 is a variant of the canonical histone H3. encoded by the H3F3A and H3F3B genes, which replaces conventional replication-dependent H3 histones (e.g., H3.1 and H3.2) in a replication-independent manner. Unlike its counterparts, H3.3 is incorporated into chromatin throughout the cell cycle, particularly at transcriptionally active regions, regulatory elements, and sites of DNA repair. This dynamic deposition is mediated by chaperones like HIRA and DAXX/ATRX, enabling H3.3 to play critical roles in epigenetic regulation, genome stability, and cellular differentiation.
Antibodies targeting Histone H3.3 are essential tools for studying its spatial and temporal distribution, post-translational modifications (PTMs), and functional roles in chromatin dynamics. These antibodies are widely used in techniques such as chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to investigate H3.3-specific epigenetic marks (e.g., H3.3K36me3) or mutations linked to diseases. For instance, somatic mutations in H3.3 (e.g., H3.3G34R/V) are associated with pediatric gliomas, while ATRX/DAXX mutations affecting H3.3 deposition correlate with alternative lengthening of telomeres (ALT) in cancers. Researchers also employ H3.3-specific antibodies to explore its involvement in developmental processes, such as reprogramming and germ cell specification. Specificity validation is critical, as cross-reactivity with other H3 variants can confound results. Overall, Histone H3.3 antibodies provide insights into chromatin biology, disease mechanisms, and potential therapeutic targets.
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