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产品描述：人髓系细胞触发受体2(TREM2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TREM2含量。TREM2是重要靶点。它参与免疫调节等生理过程。其研究机制涉及在神经炎症、肿瘤免疫等领域的作用，可调节小胶质细胞功能、影响肿瘤微环境等。对TREM2的深入研究有助于揭示相关疾病发病机制并为开发治疗方法提供方向。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于神经退行性疾病机制研究、炎症相关信号通路探索或药物干预效果评估等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TREM 2 ELISA Kit; TREM-2 ELISA Kit; TREM2 ELISA Kit; TREM2_HUMAN ELISA Kit; TREM2a ELISA Kit; TREM2b ELISA Kit; TREM2c ELISA Kit; Trggering receptor expressed on myeloid cells 2 ELISA Kit; Trggering receptor expressed on myeloid cells 2a ELISA Kit; Triggering receptor expressed on monocytes 2 ELISA Kit; Triggering receptor expressed on myeloid cells 2 ELISA Kit
缩写：TREM2
Uniprot No.：Q9NZC2
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TREM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-93 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human TREM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 98 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5022.4562.4792.366101.6681.7851.7271.61451.1151.1911.1531.0402.50.7000.6830.6920.5791.250.4640.4650.4650.3520.6250.3300.3210.3260.2130.3120.2110.2200.2160.10300.1150.1110.113 | ||||||
货期：3-5 working days
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