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产品描述：小鼠可溶性髓系细胞触发受体-1(sTREM-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TREM1含量。TREM1（髓系细胞触发受体 1）是一个重要靶点。它主要在髓系细胞表达，参与炎症反应调控。研究发现其激活后能放大炎症信号，加剧炎症反应。深入研究 TREM1 机制有助于理解炎症性疾病发病机制，为相关疾病治疗提供新方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，可为研究者开展炎症机制研究、药物疗效评估或疾病模型构建提供稳定可靠的数据支持，尤其适用于脓毒症小鼠模型、呼吸道感染模型及免疫调控相关实验的生物标志物分析。本产品仅限于科学研究使用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Trem1 ELISA Kit; Triggering receptor expressed on myeloid cells 1 ELISA Kit; TREM-1 ELISA Kit; CD antigen CD354 ELISA Kit
缩写：TREM1
Uniprot No.：Q9JKE2
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sTREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of mouse sTREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-94 | ||||
| EDTA plasma (n=4) | 100 | 91-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6572.5422.6002.4705002.0382.0892.0641.9342501.3631.4141.3891.2591250.7560.7880.7720.64262.50.4630.4540.4590.32931.30.3210.3130.3170.18715.60.2560.2660.2610.13100.1320.1280.130 | ||||||
货期：3-5 working days
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