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产品描述：人髓系细胞触发受体-1(TREM-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TREM1含量。TREM1是重要免疫调节靶点。它在炎症反应中扮演关键角色，激活后可放大炎症信号。目前研究围绕其信号通路机制展开，旨在探究如何调控其活性以干预炎症相关疾病，有望为感染性、自身免疫性等疾病治疗提供新策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外科学研究，可用于探索炎症相关疾病的分子机制、评估实验动物模型中的炎症状态或筛选调控TREM - 1表达的药物化合物，为免疫学、病理学及药理学领域的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD354 ELISA Kit; OTTMUSP00000018206 ELISA Kit; TREM 1 ELISA Kit; TREM-1 ELISA Kit; TREM1 ELISA Kit; TREM1_HUMAN ELISA Kit; Triggering receptor expressed on monocytes 1 ELISA Kit; Triggering receptor expressed on myeloid cells 1 ELISA Kit; Triggering receptor TREM 1 ELISA Kit; Triggering receptor TREM1 ELISA Kit
缩写：TREM1
Uniprot No.：Q9NP99
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 82-97 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 99-110 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-93 | |||||
回收率：
| The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-96 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5292.7522.6412.53810002.0382.0792.0591.9565001.3041.3891.3471.2442500.7010.7330.7170.6141250.4410.4550.4480.34562.50.3020.3260.3140.21131.250.2050.2010.2030.10000.1040.1010.103 | ||||||
货期：3-5 working days
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