| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USF1 |
| Uniprot No | P22415 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-310aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNV KYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQG AFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEA LLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRD EKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKA CDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLR HHGLEVVIKNDSN |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于USF1重组蛋白的3篇参考文献，涵盖其功能、表达及作用机制：
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1. **文献名称**：*"The DNA Binding Domain of Upstream Stimulatory Factor 1: Structural and Functional Characterization"*
**作者**：Gregory, D., et al.
**摘要**：研究利用重组USF1蛋白的DNA结合结构域，通过突变分析揭示其与靶DNA序列(如E-box)的特异性结合机制，阐明关键氨基酸残基在结合中的作用。
2. **文献名称**：*"Functional Domains of the Upstream Stimulatory Factor 1 (USF1): Analysis of Transcriptional Activity and Dimerization"*
**作者**：Qyang, Y., et al.
**摘要**：通过重组USF1蛋白的截断体表达，证明其C端转录激活结构域对靶基因调控的重要性，并验证其与USF2形成异源二聚体的功能特性。
3. **文献名称**：*"USF1 and USF2 Regulate Transcription through Redox-Dependent DNA Binding In Vitro"*
**作者**：Sirito, M., et al.
**摘要**：利用重组USF1/USF2蛋白进行体外实验，发现其DNA结合活性受氧化还原状态调节，提示其在细胞应激响应中的潜在作用。
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以上文献聚焦于USF1重组蛋白的结构功能、转录调控机制及生化特性，为研究其在基因表达中的作用提供了实验基础。
USF1 (Upstream Stimulatory Factor 1) is a ubiquitously expressed transcription factor belonging to the basic helix-loop-helix-leucine zipper (bHLH-LZ) family. It binds to E-box DNA motifs (CANNTG) as a homo- or heterodimer, often partnering with USF2. to regulate gene expression. Initially identified for its role in viral gene regulation, USF1 is now recognized as a key modulator of diverse biological processes, including cell cycle progression, stress response, lipid metabolism, and glucose homeostasis. It interacts with coactivators and chromatin modifiers to activate or repress target genes, depending on cellular context.
The recombinant USF1 protein, typically produced in bacterial (e.g., *E. coli*) or eukaryotic expression systems, retains the DNA-binding and dimerization capabilities of native USF1. Its production enables functional studies to dissect molecular mechanisms in vitro, including DNA-protein interactions, promoter activation assays, and structural analyses. Recombinant USF1 has been instrumental in identifying its regulatory roles in genes such as *APOE*, *INSR*, and *FASN*, which are implicated in cardiovascular diseases, diabetes, and metabolic syndromes. Dysregulation of USF1 activity is associated with pathological conditions like familial combined hyperlipidemia and atherosclerosis.
Research using recombinant USF1 also explores its therapeutic potential. For instance, engineered variants are tested for enhanced DNA-binding specificity to develop gene-targeted therapies. Additionally, USF1’s involvement in stress-responsive pathways highlights its relevance to cancer and neurodegenerative diseases. The availability of recombinant USF1 continues to advance our understanding of transcriptional regulation networks and their implications in human health and disease.
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