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产品描述：人肿瘤坏死因子受体相关因子6（TRAF6）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TRAF6含量。TRAF6 是一种重要的信号转导分子。其它参与多种细胞信号通路。研究机制上，TRAF6 在免疫调节、骨骼发育等过程中扮演关键角色，可通过激活下游信号分子，介导细胞内信号传递，引发一系列生物学效应，对相关疾病研究有重要意义。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于科研领域中对TRAF6蛋白表达水平的定量分析，例如探索其在信号通路中的调控机制、评估疾病模型中的动态变化或筛选潜在药物靶点等研究；可为免疫学、肿瘤学及分子生物学领域的体外实验提供标准化工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：E3 ubiquitin-protein ligase TRAF6 ELISA Kit; Interleukin 1 signal transducer ELISA Kit; Interleukin-1 signal transducer ELISA Kit; MGC 3310 ELISA Kit; MGC:3310 ELISA Kit; MGC3310 ELISA Kit; OTTHUMP00000232772 ELISA Kit; OTTHUMP00000232773 ELISA Kit; RING finger protein 85 ELISA Kit; RNF 85 ELISA Kit; RNF85 ELISA Kit; TNF receptor associated factor 6 ELISA Kit; TNF receptor-associated factor 6 ELISA Kit; TNF receptor-associated factor 6; E3 ubiquitin protein ligase ELISA Kit; TRAF 6 ELISA Kit; Traf6 ELISA Kit; TRAF6_HUMAN ELISA Kit
缩写：TRAF6
Uniprot No.：Q9Y4K3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRAF6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-104 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of human TRAF6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-99 | ||||
| EDTA plasma (n=4) | 90 | 86-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected25002.4442.2462.3452.23912501.9371.7801.8591.7536251.4321.5441.4881.382312.51.0781.1591.1191.0131560.7260.7600.7430.637780.3760.3580.3670.261390.2610.2440.2530.14700.1030.1090.106 | ||||||
货期：3-5 working days
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