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产品描述：人肿瘤坏死因子受体相关因子1（TRAF1）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TRAF1含量。TRAF1是一个重要靶点。它在细胞信号传导、免疫调节等过程中起关键作用。研究发现其参与调控NF - κB等信号通路，与炎症反应、肿瘤发生发展密切相关。深入研究TRAF1的作用机制，有望为相关疾病治疗提供新的靶点和策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探索TRAF1在肿瘤微环境、类风湿性关节炎等疾病模型中的表达调控机制，或用于评估药物干预、基因编辑等实验处理后TRAF1的动态变化，为信号通路研究及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：4732496E14Rik ELISA Kit; EBI6 ELISA Kit; EBV induced protein 6 ELISA Kit; Epstein-Barr virus-induced protein 6 ELISA Kit; MGC:10353 ELISA Kit; Tnf receptor associated factor 1 ELISA Kit; TNF receptor-associated factor 1 ELISA Kit; TRAF 1 ELISA Kit; TRAF1 ELISA Kit; TRAF1_HUMAN ELISA Kit
缩写：TRAF1
Uniprot No.：Q13077
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRAF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-90 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
回收率：
| The recovery of human TRAF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 86-94 | |||||
| EDTA plasma (n=4) | 107 | 103-112 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.4212.5682.4952.39710001.8581.9021.8801.7825001.2241.2771.2511.1532500.6940.7050.7000.6021250.4320.4430.4380.34062.50.2460.2520.2490.15131.250.1620.1660.1640.06600.0970.0990.098 | |||||||
货期：3-5 working days
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