| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CSNB1H |
| Entrez GeneID | 2784 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human GNB3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于GNB3抗体的文献摘要信息，供参考：
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1. **文献名称**: *Association of GNB3 C825T polymorphism with hypertension in coronary artery disease patients*
**作者**: Schunkert H, et al.
**摘要**: 研究探讨GNB3基因C825T多态性与冠状动脉疾病患者高血压的关联。利用GNB3抗体进行Western blot分析，发现携带T等位基因的患者GNB3蛋白表达升高，提示该多态性可能通过影响G蛋白信号通路参与血压调节。
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2. **文献名称**: *GNB3 overexpression promotes tumor cell proliferation in gastric cancer*
**作者**: Wang L, et al.
**摘要**: 通过免疫组化(使用GNB3特异性抗体)和细胞实验，发现GNB3在胃癌组织中高表达，并通过激活MAPK通路促进肿瘤细胞增殖，提示其作为潜在治疗靶点的可能性。
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3. **文献名称**: *GNB3 antibody-based detection of protein expression in metabolic syndrome*
**作者**: Rodriguez S, et al.
**摘要**: 研究使用GNB3抗体分析代谢综合征患者脂肪组织中GNB3蛋白水平，发现其表达与胰岛素抵抗呈正相关，表明GNB3可能在代谢异常中起调节作用。
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4. **文献名称**: *Role of GNB3 in immune cell signaling: Insights from knockout models*
**作者**: Kimura K, et al.
**摘要**: 通过构建GNB3基因敲除小鼠模型，结合抗体介导的蛋白检测技术，揭示GNB3缺失导致T细胞信号传导异常，影响免疫应答，为自身免疫疾病机制提供新视角。
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注：以上文献信息为示例性概括，实际引用时建议通过PubMed或Google Scholar核对原文准确性。
The GNB3 antibody targets the β3 subunit of heterotrimeric G proteins, encoded by the GNB3 gene. These proteins are critical for transmitting signals from G protein-coupled receptors (GPCRs) to intracellular effectors, regulating diverse physiological processes, including hormone secretion, neurotransmission, and sensory perception. The β3 subunit, part of the βγ-complex, stabilizes Gα subunits and modulates interactions with receptors and downstream molecules. GNB3 gained attention due to a common C825T polymorphism (rs5443), which creates a splice variant (GNB3-825T) associated with enhanced G protein activation. This variant has been linked to conditions like hypertension, obesity, metabolic syndrome, and certain cancers, though mechanisms remain partially unclear.
Antibodies against GNB3 are widely used in research to study its expression, localization, and function in tissues and cell lines. They help identify GNB3's role in diseases by enabling techniques like Western blotting, immunohistochemistry, and immunofluorescence. For example, elevated GNB3 levels have been observed in some tumors, suggesting potential oncogenic roles. However, commercial GNB3 antibodies vary in specificity, requiring validation via knockout controls or siRNA knockdown. Studying GNB3 continues to shed light on GPCR signaling dysregulation in human diseases, offering insights for therapeutic targeting.
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