| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Glutathione synthase; Glutathione synthetase; GSH S; GSH synthetase; GSH-S; GSHS; GSS; HEL-S-64p; HEL-S-88n |
| Entrez GeneID | 2937 |
| WB Predicted band size | Calculated MW: 52 kDa; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human Glutathione Synthetase |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于谷胱甘肽合成酶(Glutathione Synthetase, GSS)抗体的3篇参考文献及其摘要：
1. **"Development and Validation of a Polyclonal Antibody for Specific Detection of Human Glutathione Synthetase"**
- 作者：A. Tanaka et al.
- 摘要：该研究报道了一种针对人源GSS的多克隆抗体的开发与验证，通过免疫印迹(Western blot)和免疫组化(IHC)证实其特异性，并用于分析不同癌症细胞系中GSS的蛋白表达水平，发现其与氧化应激耐受性相关。
2. **"Glutathione Synthetase Deficiency: Functional Analysis Using CRISPR-Cas9 and Antibody-Based Detection"**
- 作者：M. Ristoff et al.
- 摘要：研究利用CRISPR-Cas9技术构建GSS缺陷细胞模型，结合特异性抗体探究GSS缺失对谷胱甘肽代谢的影响，发现其导致细胞内谷胱甘肽水平显著下降，并引发线粒体功能障碍。
3. **"Antibody-Based Profiling of Glutathione Synthetase in Neurodegenerative Disorders"**
- 作者：K. Patel & J. Lee
- 摘要：通过商业化GSS抗体对阿尔茨海默病和帕金森病患者脑组织样本进行分析，发现GSS表达异常与神经元氧化损伤程度相关，提示其在神经退行性疾病中的潜在病理作用。
(注：以上文献信息为模拟示例，实际引用时需查询真实数据库并核对原文。)
Glutathione synthetase (GSS) is a critical enzyme in the glutathione biosynthesis pathway, catalyzing the ATP-dependent ligation of γ-glutamylcysteine to glycine to form glutathione (GSH), a key antioxidant essential for cellular redox balance, detoxification, and immune function. Antibodies targeting GSS are valuable tools for studying its expression, localization, and regulation in various biological contexts. These antibodies are typically developed using immunogenic peptide sequences or recombinant GSS protein, enabling specific detection in techniques like Western blotting, immunohistochemistry, and immunofluorescence.
Research applications of GSS antibodies include investigating oxidative stress-related diseases (e.g., neurodegenerative disorders, diabetes, and cancer), as GSH depletion or GSS dysfunction is linked to pathological mechanisms. They also aid in diagnosing rare genetic disorders like glutathione synthetase deficiency, which causes hemolytic anemia and metabolic acidosis. Validated GSS antibodies (e.g., via knockout controls or siRNA knockdown) ensure reliability in quantifying protein levels in tissues or cell lines. Commercial availability from multiple suppliers has facilitated widespread use in both basic and clinical research, contributing to advancements in understanding redox biology and therapeutic targeting of GSH-dependent pathways.
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