| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phospholipid hydroperoxide glutathione peroxidase; mitochondrial; PHGPx; EC 1.11.1.12; Glutathione peroxidase 4; GPx-4; GSHPx-4 |
| Entrez GeneID | 2879 |
| WB Predicted band size | Calculated MW: 22 kDa; Observed MW: 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic peptide of Glutathione Peroxidase 4 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Glutathione Peroxidase 4(GPX4)抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: *Glutathione peroxidase 4 senses and translates oxidative stress into 12/15-lipoxygenase dependent- and AIF-mediated cell death*
**作者**: Seiler, A. et al. (2008)
**摘要**: 该研究揭示了GPX4通过调控脂质过氧化作用在细胞死亡中的核心作用，并开发了特异性GPX4抗体用于验证其在小鼠模型中的表达缺失与神经元退行性病变的关系。
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2. **文献名称**: *Regulation of ferroptotic cancer cell death by GPX4*
**作者**: Yang, W.S. et al. (2014)
**摘要**: 研究证实GPX4是铁死亡(ferroptosis)的关键调控因子，利用GPX4抗体通过免疫印迹和免疫荧光技术，证明其表达水平与癌细胞对铁死亡的敏感性直接相关。
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3. **文献名称**: *Inactivation of the ferroptosis regulator GPX4 triggers acute renal failure in mice*
**作者**: Friedmann Angeli, J.P. et al. (2014)
**摘要**: 通过构建GPX4基因敲除小鼠模型，结合特异性抗体检测，发现GPX4失活会导致肾小管细胞铁死亡，进而引发急性肾损伤，强调了GPX4在维持氧化还原平衡中的生理意义。
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**备注**：以上文献均涉及GPX4抗体的实验应用(如Western Blot、免疫组化)，并聚焦于GPX4在氧化应激、铁死亡及疾病机制中的功能。如需具体实验细节或抗体来源，可进一步查阅原文方法部分。
Glutathione Peroxidase 4 (GPX4) is a selenocysteine-containing enzyme critical for cellular antioxidant defense, specifically reducing lipid hydroperoxides to protect membranes from oxidative damage. As a key regulator of ferroptosis, a form of iron-dependent cell death, GPX4 has garnered significant interest in cancer, neurodegeneration, and ischemia-reperfusion injury research. Antibodies targeting GPX4 are essential tools for studying its expression, localization, and functional roles. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess GPX4 levels in tissues or cell lines under various experimental conditions, such as oxidative stress or ferroptosis induction.
GPX4 exists in multiple isoforms (cytosolic, mitochondrial, nuclear), and antibody specificity depends on epitope recognition. Many commercially available GPX4 antibodies are raised against conserved regions, enabling cross-reactivity in human, mouse, and rat samples. Validation often includes knockout cell controls to confirm target specificity. Researchers frequently employ these antibodies to investigate GPX4 downregulation in disease models or its therapeutic modulation. Recent studies also explore GPX4 as a biomarker for therapy resistance, particularly in cancers where ferroptosis evasion contributes to progression. However, interpretation requires caution due to GPX4's low abundance and potential post-translational modifications affecting antibody binding.
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