| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gen |
| Entrez GeneID | 348654 |
| WB Predicted band size | Calculated MW: 103 kDa; Observed MW: 103 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human GEN1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于GEN1抗体的虚构参考文献示例(内容为假设性描述)：
---
1. **文献名称**：Structural insights into GEN1 endonuclease activity through antibody-mediated crystallization
**作者**：Thompson R, et al.
**摘要**：本研究利用特异性GEN1单克隆抗体促进蛋白质结晶，解析了GEN1核酸酶的三维结构，揭示了其切割Holliday junctions的分子机制，为靶向DNA修复的疗法提供理论依据。
2. **文献名称**：GEN1 expression profiling in ovarian cancer using immunohistochemical analysis
**作者**：Chen L, et al.
**摘要**：通过开发高特异性GEN1多克隆抗体，检测卵巢癌组织中GEN1蛋白表达水平，发现其与肿瘤基因组不稳定性及化疗耐药性显著相关，提示其作为预后生物标志物的潜力。
3. **文献名称**：Functional disruption of GEN1 in mouse models via antibody-guided CRISPR inhibition
**作者**：Park S, et al.
**摘要**：利用GEN1抗体靶向递送CRISPR抑制剂至小鼠胚胎细胞，成功实现GEN1基因的条件性敲除，验证了其在胚胎发育中维持染色体完整性的关键作用。
4. **文献名称**：Comparative study of commercial GEN1 antibodies for specificity validation in Western blotting
**作者**：Müller J, et al.
**摘要**：系统评估了5种市售GEN1抗体的交叉反应性和灵敏度，推荐克隆号#7C2的抗体在Western blot中表现最优，为后续研究提供可靠的实验工具选择。
---
注：以上文献为模拟内容，实际引用需以真实出版物为准。
**Background of GEN1 Antibody**
The GEN1 antibody targets the GEN1 protein, a structure-selective endonuclease critical in DNA repair and genome stability. GEN1 (also called GEN1 or Yen1 in yeast) is a member of the Rad2/XPG nuclease family and plays a key role in resolving DNA recombination intermediates, particularly Holliday junctions (HJs), during homologous recombination (HR). Unlike other HJ resolvases, such as MUS81-EME1 or SLX1-SLX4. GEN1 operates independently in the nucleus during the late stages of mitosis, ensuring faithful chromosome segregation.
First identified in *Caenorhabditis elegans*, GEN1 is evolutionarily conserved and shares functional homology with human and yeast orthologs. Its endonuclease activity cleaves HJs symmetrically, preventing DNA damage and chromosomal abnormalities. Dysregulation or mutations in GEN1 are linked to genomic instability, cancer predisposition, and developmental disorders.
GEN1 antibodies are essential tools in studying DNA repair mechanisms, enabling detection of GEN1 expression, localization, and interactions via techniques like Western blotting, immunofluorescence, or chromatin immunoprecipitation. Research using these antibodies has highlighted GEN1's role in backup repair pathways when primary resolvases fail, offering insights into cancer therapy resistance and synthetic lethality strategies targeting DNA repair deficiencies.
Overall, GEN1 antibodies contribute to understanding genome maintenance, with implications for oncology and genetic disease research.
×
