| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GCN5; hGCN5; GCN5L2; PCAF-b |
| Entrez GeneID | 2648 |
| WB Predicted band size | Calculated MW: 94 kDa; Observed MW: 94 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant protein of human GCN5 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于KAT2A抗体的3篇代表性文献(虚构示例，仅供参考格式)：
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1. **文献名称**：*KAT2A-mediated acetylation switches the metabolic response in hepatocellular carcinoma*
**作者**：Zhang, Y. et al.
**摘要**：通过KAT2A抗体进行染色质免疫沉淀(ChIP)和免疫印迹分析，揭示了KAT2A通过乙酰化代谢相关转录因子，调控肝癌细胞糖酵解和氧化磷酸化的平衡。
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2. **文献名称**：*A functional screen identifies KAT2A as a critical regulator of glioblastoma stemness*
**作者**：Lee, S. et al.
**摘要**：利用KAT2A抗体进行免疫荧光和Western blot，证明KAT2A通过乙酰化SOX2维持胶质母细胞瘤干性，其抑制可显著减少肿瘤球形成能力。
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3. **文献名称**：*KAT2A antibody validation for epigenetic studies in human fibroblasts*
**作者**：Martinez, R. et al.
**摘要**：系统评估了KAT2A抗体在ChIP-seq和免疫沉淀中的特异性，确认其适用于检测内源性KAT2A蛋白定位及相互作用网络，为表观遗传研究提供工具验证。
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(注：以上文献为示例，实际引用需查询真实数据库如PubMed，并核对作者、标题及摘要内容。)
**Background of KAT2A Antibody**
KAT2A (lysine acetyltransferase 2A), also known as GCN5. is a critical enzyme in the GNAT (Gcn5-related N-acetyltransferase) family that catalyzes histone acetylation, a key epigenetic modification regulating chromatin structure and gene expression. By transferring acetyl groups to lysine residues on histones (e.g., H3K9. H4K14), KAT2A facilitates chromatin relaxation, promoting transcriptional activation. It is involved in diverse cellular processes, including cell cycle progression, metabolism, differentiation, and DNA repair, often functioning within multiprotein complexes like STAGA and ATAC.
KAT2A-specific antibodies are essential tools for studying its role in epigenetic regulation. They are widely used in techniques such as chromatin immunoprecipitation (ChIP), Western blotting, immunofluorescence, and co-immunoprecipitation to map KAT2A binding sites, assess protein expression, or investigate interactions with cofactors. Dysregulation of KAT2A has been linked to cancers, neurodevelopmental disorders, and metabolic diseases, making its antibody valuable in both basic and translational research.
Validated KAT2A antibodies typically target conserved regions, such as the N-terminal domain, and are tested for specificity using knockout controls or siRNA-mediated depletion. Cross-reactivity with paralogs (e.g., KAT2B/PCAF) must be ruled out. Commercial antibodies are often verified across human, mouse, or rat samples, enabling comparative studies in disease models. Reliable KAT2A antibodies are critical for elucidating its context-dependent roles in health and disease.
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