| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AI448320; AW552119 |
| Entrez GeneID | 14360 |
| WB Predicted band size | Calculated MW: 61 kDa; Observed MW: 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of mouse Fyn |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于FYN抗体的3篇参考文献示例(内容为模拟，仅供参考)：
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1. **文献名称**：FYN Kinase in Alzheimer's Disease: Role in Aβ Oligomer-Induced Synaptic Dysfunction
**作者**：Smith J, et al.
**摘要**：本研究利用FYN特异性抗体，通过免疫印迹和免疫组织化学技术，揭示了FYN激酶在阿尔茨海默病模型小鼠海马区异常激活的现象。实验表明，抑制FYN活性可改善Aβ寡聚体诱导的突触功能损伤，提示其作为潜在治疗靶点。
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2. **文献名称**：FYN Regulates Epithelial-Mesenchymal Transition in Breast Cancer via STAT3 Signaling
**作者**：Li X, Wang Y, et al.
**摘要**：通过FYN抗体介导的免疫共沉淀技术，研究发现FYN与STAT3在乳腺癌细胞中直接相互作用，促进上皮-间质转化(EMT)。使用FYN抑制剂或抗体敲低FYN表达后，肿瘤细胞迁移能力显著降低。
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3. **文献名称**：Development of a High-Affinity Monoclonal Antibody Against Human FYN for Diagnostic Applications
**作者**：Garcia R, et al.
**摘要**：本研究报道了一种新型抗人FYN单克隆抗体的开发，该抗体在ELISA和流式细胞术中表现出高特异性和灵敏度。实验验证其在检测结直肠癌患者组织样本中FYN过表达的临床应用潜力。
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4. **文献名称**：FYN Antibody-Based CRISPR Screening Identifies T Cell Receptor Signaling Modulators
**作者**：Chen L, et al.
**摘要**：结合FYN抗体富集技术和CRISPR-Cas9筛选，系统性分析了T细胞受体(TCR)信号通路中FYN的调控网络，发现多个未知的互作蛋白，为免疫治疗提供了新靶点。
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注：以上为模拟文献，实际引用需查询具体数据库(如PubMed/Web of Science)。
FYN antibody is a research tool targeting FYN, a non-receptor tyrosine kinase belonging to the SRC family. FYN plays critical roles in cellular signaling, particularly in T-cell receptor (TCR) signaling, neuronal development, and synaptic plasticity. It is encoded by the *FYN* gene, producing multiple isoforms through alternative splicing, with the neuronal isoform prominently expressed in the brain. Structurally, FYN contains SH2 and SH3 domains for protein interactions and a kinase domain for phosphorylation activity.
In immunology, FYN is essential for TCR signal transduction, regulating immune cell activation and differentiation. In neuroscience, it interacts with postsynaptic density proteins, modulating synaptic transmission and memory formation. Notably, FYN phosphorylates tau protein, linking it to Alzheimer’s disease pathology by promoting neurofibrillary tangle formation. Dysregulated FYN activity is also implicated in cancers, such as melanoma and glioblastoma, where it drives proliferation and metastasis.
FYN antibodies are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to detect FYN expression, localization, and activation (e.g., phosphorylation at Tyr420). They aid in studying FYN’s dual roles in disease—both as a pathogenic driver and a potential therapeutic target. Recent studies explore FYN inhibitors for neurodegenerative disorders and immune modulation, highlighting its translational relevance. Commercial FYN antibodies vary in specificity, often distinguishing isoforms or phosphorylation states, necessitating validation for experimental contexts.
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