| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FTO; KIAA1752; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein |
| Entrez GeneID | 79068 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human FTO |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及FTO抗体的参考文献示例(注：文献为模拟示例，具体内容请根据实际研究补充)：
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1. **文献名称**: "Development of a High-Specificity FTO Antibody for m6A Demethylation Studies"
**作者**: Li, X. et al.
**摘要**: 本研究报道了一种新型兔源多克隆FTO抗体的开发，通过重组人FTO蛋白免疫制备，验证了其在Western blot和免疫荧光中的特异性。该抗体成功用于检测细胞中FTO蛋白表达及其与RNA m6A去甲基化功能的关联。
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2. **文献名称**: "FTO Antibody-Based Detection of Obesity-Related Protein in Human Adipose Tissue"
**作者**: Wang, Y. & Zhang, H.
**摘要**: 通过商业化FTO单克隆抗体(货号AB123.ABC公司)，研究者分析了肥胖患者脂肪组织中FTO蛋白的表达水平，发现其与BMI呈正相关。研究强调了抗体在临床样本免疫组化中的可靠性。
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3. **文献名称**: "FTO Inhibition via Antibody Targeting Suppresses Cancer Cell Proliferation"
**作者**: Chen, L. et al.
**摘要**: 利用FTO特异性抗体阻断其酶活性，研究证明在胶质母细胞瘤模型中，FTO功能抑制可降低癌细胞的m6A修饰水平并抑制肿瘤生长，为靶向FTO的抗体治疗提供了实验依据。
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建议通过PubMed或Google Scholar以关键词“FTO antibody”、“FTO protein detection”进一步检索最新文献。
The FTO (Fat Mass and Obesity-Associated) protein, encoded by the FTO gene, is a member of the AlkB family of non-heme Fe(II)/α-ketoglutarate-dependent dioxygenases. Initially linked to obesity through genome-wide association studies, FTO gained attention for its role in regulating energy homeostasis and metabolism. It functions as an RNA demethylase, primarily targeting N6-methyladenosine (m6A) and other modifications in mRNA, influencing RNA splicing, stability, and translation. This epigenetic regulation impacts genes involved in adipogenesis, thermogenesis, and neuronal signaling.
FTO antibodies are critical tools for studying its expression, localization, and molecular interactions. They enable detection via techniques like Western blot, immunohistochemistry, and immunofluorescence, helping researchers explore FTO's tissue-specific roles. Dysregulation of FTO is associated with metabolic disorders (e.g., diabetes), cancer (e.g., glioblastoma, leukemia), and neurological conditions, making it a potential therapeutic target. Antibodies also aid in investigating FTO inhibitors, which show promise in preclinical studies for metabolic and oncological interventions.
Research using FTO antibodies has expanded understanding of its dual role in physiology and disease, bridging epigenetics, metabolism, and cellular signaling. These studies underscore FTO's complexity, as its effects vary by cellular context and disease stage, highlighting the need for precise experimental models and antibody specificity in biomedical research.
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