| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FTO; KIAA1752; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein |
| Entrez GeneID | 79068 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human FTO |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与FTO抗体相关的文献摘要概览：
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1. **"FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing"**
- Jia, G. et al. (2011)
- **摘要**：研究揭示了FTO蛋白通过催化RNA中N6-甲基腺苷(m6A)的去甲基化调控mRNA剪接。实验中使用特异性FTO抗体进行Western blot和免疫荧光，证实FTO在细胞核内的定位及其与剪接因子的相互作用。
2. **"FTO suppresses glycolysis and growth of papillary thyroid cancer via decreasing stability of APOE mRNA in an N6-methyladenosine-dependent manner"**
- Cui, Y. et al. (2022)
- **摘要**：探讨FTO在甲状腺癌中的抑癌作用，发现其通过m6A修饰降低APOE mRNA稳定性抑制糖酵解。研究利用FTO抗体进行免疫组化，显示FTO低表达与患者不良预后相关，并通过敲除实验验证机制。
3. **"The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase"**
- Gerken, T. et al. (2007)
- **摘要**：首次鉴定FTO蛋白为依赖2-酮戊二酸的核酸去甲基酶，奠定其与肥胖的分子关联。通过FTO抗体在小鼠组织中检测蛋白表达，发现其广泛分布于下丘脑等代谢调控关键区域，提示其生理功能。
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这些研究均涉及FTO抗体的应用(如蛋白检测、定位或疾病分析)，涵盖分子机制、癌症代谢及肥胖关联等领域。如需扩展，可进一步检索近年文献以补充最新进展。
The FTO (fat mass and obesity-associated) protein, encoded by the FTO gene, is a nucleic acid demethylase primarily involved in regulating RNA modification and epigenetic signaling. Initially linked to obesity through genome-wide association studies, FTO gained attention for its role in energy homeostasis and metabolic regulation. It catalyzes the removal of methyl groups from N6-methyladenosine (m6A) in RNA, influencing mRNA splicing, stability, and translation. Dysregulation of FTO has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a focus of biomedical research.
FTO antibodies are essential tools for studying the protein's expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercially available FTO antibodies target specific epitopes, often validated in knockout models to ensure specificity. However, challenges remain due to potential cross-reactivity with homologous proteins (e.g., ALKBH5) or isoforms. Researchers use these antibodies to explore FTO's tissue-specific roles, its interaction with metabolic pathways, and its therapeutic potential as a drug target. Recent studies also investigate its involvement in viral infections and immune responses, expanding its relevance beyond metabolic diseases. Validation of antibody performance remains critical for data reproducibility in FTO-related research.
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