| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UQCRH |
| Uniprot No | P07919 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 14-91aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSGDPEEEEEEEEELVDPLTTVREQCEQL EKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNL K |
| 预测分子量 | 12 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UQCRH重组蛋白的示例参考文献(注：以下文献为虚构示例，仅用于演示格式，非真实存在)：
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1. **文献名称**: *Expression and functional characterization of recombinant human UQCRH in mitochondrial complex III*
**作者**: Zhang L, et al.
**摘要**: 本研究在大肠杆菌系统中成功表达并纯化了重组人源UQCRH蛋白，验证其在线粒体复合物III中的结构稳定性，并发现其通过调节活性氧(ROS)影响细胞凋亡通路。
2. **文献名称**: *UQCRH mutations impair mitochondrial respiration and correlate with metabolic disorders*
**作者**: Kim S, et al.
**摘要**: 通过构建UQCRH突变体重组蛋白，揭示其导致线粒体复合物III功能缺陷，降低ATP合成效率，与家族性代谢综合征患者的临床表现相关联。
3. **文献名称**: *UQCRH as a potential biomarker in colorectal cancer: Recombinant protein-based analysis*
**作者**: Müller R, et al.
**摘要**: 利用重组UQCRH蛋白进行高通量筛选，发现其在结直肠癌组织中表达显著下调，并通过调控线粒体膜电位抑制肿瘤细胞增殖。
4. **文献名称**: *Structural insights into UQCRH-mediated electron transfer by cryo-EM*
**作者**: Watanabe K, et al.
**摘要**: 结合冷冻电镜技术与重组UQCRH蛋白，解析其在复合物III中的精确空间构象，阐明其在电子传递链中的关键作用及与神经退行性疾病的潜在联系。
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建议通过PubMed、Web of Science等平台检索真实文献，关键词可包括 **UQCRH recombinant protein**、**UQCRH complex III**、**UQCRH disease**。
UQCRH (Ubiquinol-Cytochrome c Reductase Hinge Protein) is a nuclear-encoded subunit of mitochondrial Complex III (cytochrome *bc*1 complex), a critical component of the electron transport chain (ETC) responsible for oxidative phosphorylation. This 11.5 kDa protein anchors the Rieske iron-sulfur protein (UQCRFS1) and cytochrome *c*1 within Complex III, facilitating electron transfer from ubiquinol to cytochrome *c*. Its hinge-like structural role ensures proper conformational changes during the Q-cycle mechanism, optimizing proton pumping and ATP synthesis. UQCRH contains a transmembrane domain and conserved residues for heme binding and protein-protein interactions.
Recombinant UQCRH proteins are engineered using expression systems like *E. coli* or mammalian cells to study its biochemical properties, structural dynamics, and pathological relevance. Researchers employ techniques such as site-directed mutagenesis to probe functional domains or disease-linked mutations. Dysregulation of UQCRH is implicated in cancers, neurodegenerative disorders, and mitochondrial diseases due to its impact on ETC efficiency, ROS production, and apoptosis. For example, UQCRH overexpression in some tumors correlates with enhanced proliferation via metabolic reprogramming, while its deficiency may impair neuronal energy metabolism.
Recombinant UQCRH also aids in antibody development for diagnostic assays and crystallography studies to resolve Complex III architecture. Its production enables functional rescue experiments in cellular models with mitochondrial dysfunction. Despite its small size, UQCRH’s integrative role in ETC mechanics and disease pathways makes it a focal point for investigating mitochondrial disorders and therapeutic strategies targeting bioenergetic pathways. Current research explores its interactions with ETC inhibitors/activators and potential as a biomarker for mitochondrial health.
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