| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Flotillin-1 |
| Entrez GeneID | 10211 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Flotillin 1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于Flotillin 1抗体的3篇参考文献及其摘要内容：
1. **文献名称**: "Flotillin-1 is a novel biomarker for the progression of melanoma"
**作者**: Hazarika P et al.
**摘要**: 该研究通过免疫组化实验，发现Flotillin 1在黑色素瘤转移组织中高表达，且其抗体检测结果与肿瘤侵袭性显著相关，提示Flotillin 1可作为癌症进展的生物标志物。
2. **文献名称**: "Flotillins regulate membrane dynamics and cellular signaling through lipid raft association"
**作者**: Glebov OO et al.
**摘要**: 文章利用Flotillin 1特异性抗体进行免疫荧光和Western blot分析，发现Flotillin 1通过脂筏结构参与细胞膜重塑和信号转导，尤其在EGFR等受体酪氨酸激酶的激活中起关键作用。
3. **文献名称**: "Flotillin-1 interacts with the amyloid precursor protein and regulates its trafficking in neurons"
**作者**: Schulte T et al.
**摘要**: 通过共聚焦显微镜和免疫沉淀实验(使用Flotillin 1抗体)，该研究揭示了Flotillin 1与淀粉样前体蛋白(APP)的相互作用，影响APP在神经元中的运输及Aβ肽段的生成，可能与阿尔茨海默病病理相关。
4. **文献名称**: "Antibody-based profiling of flotillin-1 expression in human tissues"
**作者**: Ludwig A et al.
**摘要**: 该文献系统性评估了Flotillin 1抗体在不同组织样本中的特异性和灵敏度，验证了其在癌症、神经疾病等研究中的可靠性，并提供了标准化实验条件建议。
Flotillin-1 (FLOT1) is a conserved, ubiquitously expressed protein belonging to the flotillin family, which includes Flotillin-1 and Flotillin-2. These proteins are integral components of lipid raft microdomains in cellular membranes, playing critical roles in membrane organization, vesicle trafficking, and signal transduction. Flotillin-1 is encoded by the *FLOT1* gene in humans and is involved in processes such as endocytosis, cell adhesion, and receptor tyrosine kinase signaling. It is also implicated in pathological conditions, including cancer progression, neurodegeneration, and viral infections, where its dysregulation often correlates with disease severity.
Antibodies targeting Flotillin-1 are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate lipid raft dynamics and protein-protein interactions. Flotillin-1 antibodies help identify its role in cellular processes, such as membrane remodeling during cell division or pathogen entry. In cancer research, these antibodies are employed to assess Flotillin-1 overexpression in tumors, which has been linked to enhanced metastasis and poor prognosis. Validation of antibody specificity is crucial, as cross-reactivity with Flotillin-2 or other membrane-associated proteins can occur. Overall, Flotillin-1 antibodies serve as vital reagents for exploring both physiological and pathological mechanisms involving lipid raft-associated signaling.
×
